Hostname: page-component-cd9895bd7-jkksz Total loading time: 0 Render date: 2024-12-28T20:24:24.387Z Has data issue: false hasContentIssue false
  • English
  • Français

Development of a novel PCR assay capable of detecting a single Schistosoma japonicum cercaria recovered from Oncomelania hupensis

Published online by Cambridge University Press:  10 June 2005

A. J. DRISCOLL ,
J. L. KYLE  and
J. REMAIS
A. J. DRISCOLL
Affiliation:
Center for Occupational and Environmental Health, School of Public Health, University of California at Berkeley, 140 Warren Hall #7360, Berkeley, CA 94720, USA
J. L. KYLE
Affiliation:
Graduate Group in Microbiology, Department of Plant and Microbial Biology, University of California at Berkeley, 111 Koshland Hall, Berkeley, CA 94720, USA Division of Infectious Diseases, School of Public Health, University of California at Berkeley, 140 Warren Hall, Berkeley, CA 94720, USA
J. REMAIS
Affiliation:
Center for Occupational and Environmental Health, School of Public Health, University of California at Berkeley, 140 Warren Hall #7360, Berkeley, CA 94720, USA
Get access Rights & Permissions [Opens in a new window]

Abstract

Location and time-specific variability in Schistosoma japonicum cercarial density has been shown to be high in the mountainous regions of Sichuan Province, China. A polymerase chain reaction (PCR) assay for the detection of schistosome cercariae in these environments would aid in the determination of environmental risk, and the identification of individual-level risk factors. Here the authors present a highly sensitive and specific PCR assay for the detection of S. japonicum cercariae in laboratory samples. As few as 1 and as many as 300 cercariae, from both laboratory and field-collected S. japonicum strains, produced positive amplification results, and repeated assays showed no positive result for S. mansoni nor for non-japonicum cercariae isolated from infected snails collected in Sichuan Province. There was no difference found between the Chinese and Philippine S. japonicum strains. The results presented demonstrate the successful PCR amplification of a target sequence within the SjR2 retrotransposon from samples of S. japonicum cercariae, with the potential for application to natural water samples from endemic areas.

Keywords

Schistosoma japonicumcercariaepolymerase chain reactiondetectioncercariometryspecificitysensitivity
Type
Research Article
Information
Parasitology , Volume 131 , Issue 4 , October 2005 , pp. 497 - 500
Copyright
© 2005 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Hamburger, J., Xu, Y. X., Ramzy, R. M., Jourdane, J. and Ruppel, A. ( 1998). Development and laboratory evaluation of a polymerase chain reaction for monitoring Schistosoma mansoni infestation of water. American Journal of Tropical Medicine and Hygiene 59, 468–473.CrossRefGoogle Scholar
Hertel, J., Hamburger, J., Haberl, B. and Haas, W. ( 2002). Detection of bird schistosomes in lakes by PCR and filter-hybridization. Experimental Parasitology 101, 57–63.CrossRefGoogle Scholar
Laha, T., Brindley, P. J., Smout, M. J., Verity, C. K., McManus, D. P. and Loukas, A. ( 2002). Reverse transcriptase activity and untranslated region sharing of a new RTE-like, non-long terminal repeat retrotransposon from the human blood fluke, Schistosoma japonicum. International Journal for Parasitology 32, 1163–1174.CrossRefGoogle Scholar
Rivas, R., Velazquez, E., Zurdo-Pineiro, J. L., Mateos, P. F. and Monlina, E. M. ( 2003). Identification of microorganisms by PCR amplification and sequencing of a universal amplified ribosomal region present in both prokaryotes and eukaryotes. Journal of Microbiological Methods 56, 413–426.Google Scholar
Spear, R. C., Seto, E., Liang, S., Birkner, M., Hubbard, A., Qui, D., Yang, C., Zhong, B., Xu, F., Gu, X. and Davis, G. M. ( 2004 a). Factors influencing the transmission of Schistosoma japonicum in the mountains of Sichuan province. American Journal of Tropical Medicine and Hygiene 70, 48–56.Google Scholar
Spear, R. C., Bo, Z., Yong, M., Hubbard, A., Birkner, M., Remais, J. and Dongchaun, Q. ( 2004 b). Spatial and temporal variability in schistosome cercarial density detected by mouse bioassays in village irrigation ditches in Sichuan, China. American Journal of Tropical Medicine and Hygiene 71, 554–555.Google Scholar
Zong, B., Xiong, M. E., Zhao, L. G., Lai, Y. H., Lai, J., Chen, L., Yin, H. Z., Sha, K. Y., Zhang, Y. and Gu, X. G. ( 2001). Investigation of water contamination situation in Qionghai Lake area, Xichang, Sichuan. Journal of Practical Parasitic Diseases 9, 97–100.Google Scholar