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Development and ultrastructure of Trachipleistophora hominis n.g., n.sp. after in vitro isolation from an AIDS patient and inoculation into athymic mice

Published online by Cambridge University Press:  06 April 2009

W. S. Hollister ,
E. U. Canning ,
E. Weidner ,
A. S. Field ,
J. Kench  and
D. J. Marriott
W. S. Hollister
Affiliation:
Department of Biology, Imperial College of Science, Technology and Medicine, London SW7 2AZ, UK
E. U. Canning
Affiliation:
Department of Biology, Imperial College of Science, Technology and Medicine, London SW7 2AZ, UK
E. Weidner
Affiliation:
Department of Zoology and Physiology, Louisiana State University, Baton Rouge, Louisiana 70803–1725, USA
A. S. Field
Affiliation:
St Vincent's Hospital, Darlinghurst, Sydney, NSW 2010, Australia
J. Kench
Affiliation:
St Vincent's Hospital, Darlinghurst, Sydney, NSW 2010, Australia
D. J. Marriott
Affiliation:
St Vincent's Hospital, Darlinghurst, Sydney, NSW 2010, Australia
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Summary

Continuous culture was achieved in several cell lines of a microsporidium obtained from the skeletal muscle of an AIDS patient. Development in COS-1 and RK13 cells was prolific. Spores from the original biopsy were also inoculated into athymic mice by i.m. and i.p. routes. Infection was found in several organs as well as in skeletal muscle after a few weeks. All stages were surrounded by an electron-dense surface coat. Meronts had 2–4 nuclei and divided by binary fission. In sporogony the surface coat became separated from the plasma membrane to form a sporophorous vesicle, within which division into sporoblasts was effected by repeated binary fissions. The number of sporoblasts (and later spores) within the sporophorous vesicles varied from 2 to > 32 and the sizes of the vesicles varied, according to the number of spores contained therein, from 5 μm diameter to 14·0 × 11·0 μm. Spores measured 4·0 × 2·4 μm and had a prominent posterior vacuole. The parasite differs from the genus Pleistophora in that it does not form multinucleate sporogonial plasmodia and that the sporophorous vesicle enlarges during sporogony and its wall is not a multilayered structure. It is proposed to place it in a new genus and species Trachipleistophora hominis n.g., n.sp.

Keywords

Trachipleistophora hominis n.g., n.sp.skeletal muscleAIDS patientin vitro cultureathymic miceexperimental infection.
Type
Research Article
Information
Parasitology , Volume 112 , Issue 1 , January 1996 , pp. 143 - 154
Copyright
Copyright © Cambridge University Press 1996

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