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Determination of CD4+ and CD8+ T cells in the peripheral blood of dogs with demodicosis

Published online by Cambridge University Press:  12 July 2010

S. K. SINGH ,
U. DIMRI ,
M. C. SHARMA ,
B. SHARMA  and
M. SAXENA
S. K. SINGH*
Affiliation:
Division of Medicine, Indian Veterinary Research Institute, Izatnagar-243 122, Bareilly, U.P., India
U. DIMRI
Affiliation:
Division of Medicine, Indian Veterinary Research Institute, Izatnagar-243 122, Bareilly, U.P., India
M. C. SHARMA
Affiliation:
Division of Medicine, Indian Veterinary Research Institute, Izatnagar-243 122, Bareilly, U.P., India
B. SHARMA
Affiliation:
Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar-243 122, Bareilly, U.P., India
M. SAXENA
Affiliation:
Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar-243 122, Bareilly, U.P., India
*
*Corresponding author: Division of Medicine, Indian Veterinary Research Institute, Izatnagar-243 122, Bareilly, U.P., India. Tel: +91 581 2300587. Fax: +91 581 2303284. E-mail: [email protected]
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Summary

The aim of this study was to evaluate the CD4+/CD8+ ratio in peripheral blood of dogs with localized and generalized demodicosis. Sixteen dogs were examined, 8 with localized and 8 with generalized demodicosis, while 8 healthy dogs were used as controls. Peripheral blood was obtained and CD4+ and CD8+ T cells were determined by flow cytometry. Significantly higher numbers of CD8+ T cells and lower numbers of CD4+ T cells were found in dogs with generalized demodicosis compared to dogs with localized demodicosis and healthy controls. Significantly higher numbers of CD8+ T cells and lower numbers of CD4+ T cells were also found in dogs with localized demodicosis compared to healthy controls. The CD4+/CD8+ ratio was also found to be significantly lower in dogs with generalized demodicosis in comparison with dogs with localized demodicosis and healthy controls. It is concluded that significant alteration in the CD4+/CD8+ ratio may be implicated in the pathogenesis of generalized canine demodicosis.

Keywords

CD8+ T cellsCD4+ T cellscanine demodicosisperipheral blood
Type
Research Article
Information
Parasitology , Volume 137 , Issue 13 , November 2010 , pp. 1921 - 1924
Copyright
Copyright © Cambridge University Press 2010

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