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Detection of Leishmania lipophosphoglycan binding proteins in the gut of the sandfly vector

Published online by Cambridge University Press:  01 January 1999

R. J. DILLON  and
R. P. LANE
R. J. DILLON
Affiliation:
Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath BA2 7AY
R. P. LANE
Affiliation:
The Wellcome Trust, 183 Euston Rd, London NW1 2BE
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Abstract

Binding to the midgut microvillar surface in the sandfly Phlebotomus papatasi is a prerequisite for successful development of Leishmania major within the gut of the vector. This paper describes a method for detecting microvillar-associated proteins which act as ligands for the parasite surface glycoconjugate lipophosphoglycan (LPG). Adhesion of LPG to midgut proteins was visualized by probing midgut extracts with LPG using a Western ligand blotting technique. Procyclic L. major LPG bound to a microvillar polypeptide band of 65 kDa (estimated in the non-reduced state) and bound variably to several lower molecular weight bands, probably degradation products or subunits of the primary binding polypeptides. Specificity of binding was confirmed by co-incubating biotinylated LPG with an LPG-specific mAb which resulted in a great reduction in binding.

Keywords

Leishmania majorlipophosphoglycanPhlebotomus papatasimicrovillimidgut
Type
Research Article
Information
Parasitology , Volume 118 , Issue 1 , January 1999 , pp. 27 - 32
Copyright
1999 Cambridge University Press

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