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Description of Sarcocystis turdusi sp. nov. from the common blackbird (Turdus merula)

Published online by Cambridge University Press:  20 July 2012

L. KUTKIENĖ ,
P. PRAKAS ,
D. BUTKAUSKAS  and
A. SRUOGA
L. KUTKIENĖ
Affiliation:
Nature Research Centre, Institute of Ecology, Laboratory of Molecular Ecology, Akademijos 2, LT-08412 Vilnius, Lithuania
P. PRAKAS*
Affiliation:
Nature Research Centre, Institute of Ecology, Laboratory of Molecular Ecology, Akademijos 2, LT-08412 Vilnius, Lithuania
D. BUTKAUSKAS
Affiliation:
Nature Research Centre, Institute of Ecology, Laboratory of Molecular Ecology, Akademijos 2, LT-08412 Vilnius, Lithuania
A. SRUOGA
Affiliation:
Vytautas Magnus University, Faculty of Natural Sciences, Department of Biology, K. Donelaičio 58, LT-44248 Kaunas, Lithuania
*
*Corresponding author: Nature Research Centre, Institute of Ecology, Laboratory of Molecular Ecology, Vilnius, Lithuania. Tel: +370 6 7185123. Fax: +370 5 2729352. E-mail: [email protected]
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Summary

Cysts of Sarcocystis species were found in 24 of 44 (54·5%) examined blackbirds (Turdus merula). Under the light microscope, only 1 morphological type of cyst was found in all birds investigated. Ribbon-shaped cysts were long (the largest fragment found amounted to 7 mm) and of different thickness (25–206 μm). A cyst wall reached up to 3·5 μm and had finger-like protrusions. Under the transmission electron microscope, a single cyst isolated from 1 blackbird was studied. The cyst wall was 2·5–4·4 μm thick, had club- or irregularly-shaped and sometimes branched protrusions that differed in size. The content of cysts was divided into large chambers by septa. Orange segment-shaped cystozoites were 6·2 × 1·4 (5·5–7·2 × 1·2–1·5) μm. This type of cyst wall has never been described in Sarcocystis species isolated from birds, thus far. The results of 18S rDNA, 28S rDNA and ITS–1 region sequences showed that S. turdusi was most closely related to S. columbae, S. calchasi, S. wobeseri, S. cornixi and Sarcocystis sp. ex Accipiter nisus parasitizing birds. Phylogenetic results suggest that predatory birds are the most probable definitive hosts of S. turdusi.

Keywords

Sarcocystisblackbirdcyst morphology18S rDNA28S rDNAITS–1
Type
Research Article
Information
Parasitology , Volume 139 , Issue 11 , September 2012 , pp. 1438 - 1443
Copyright
Copyright © Cambridge University Press 2012

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References

REFERENCES

Box, E. D., Meier, J. L. and Smith, J. H. (1984). Description of Sarcocystis falcatula Stiles, 1893, a parasite of birds and opossums. Journal of Protozoology 31, 521–524. doi: 10.1111/j.1550-7408.1984.tb05495.xCrossRefGoogle ScholarPubMed
Brumpt, E. (1913). Precis de Parasitologie, 3rd Edn.Masson et Compagnie, Paris.Google Scholar
Bütschli, O. (1880–82). Protozoa. Sarcosporidia. In Klassen und Ordnungen des Tier-Reichs Vol. 1 (ed. Bronn, H. G.) pp. 604–616. C. F. Winter'sche Verlagshandlung, Leipzig and Seidelberg, Germany.Google Scholar
Černá, Ž. (1984). The role of birds as definitive hosts and intermediate hosts of heteroxenous coccidians. Journal of Protozoology 31, 579–581. doi: 10.1111/j.1550-7408.1984.tb05508.xCrossRefGoogle ScholarPubMed
Dahlgren, S. S., Gouveia-Oliveira, R. and Gjerde, B. (2008). Phylogenetic relationships between Sarcocystis species from reindeer and other Sarcocystidae deduced from ssu rRNA gene sequences. Veterinary Parasitology 151, 27–35. doi:10.1016/j.vetpar.2007.09.029.CrossRefGoogle ScholarPubMed
Doležel, D., Koudela, B., Jirků, M., Hypša, V., Oborník, M., Votýpka, J., Modrý, D., Šlapeta, J. R. and Lukeš, J. (1999). Phylogenetic analysis of Sarcocystis spp. of mammals and reptiles supports the coevolution of Sarcocystis spp. with their final hosts. International Journal for Parasitology 29, 795–798. doi: 10.1016/j.bbr.2011.03.031.CrossRefGoogle ScholarPubMed
Dubey, J. P. and Odening, K. (2001). Toxoplasmosis and related infections. In Parasitic Diseases of Wild Mammals (ed. Samuel, W. M., Pybus, M. J. and Kocan, A. A.). pp. 478–519. Iowa State University Press, Ames, IO, USA.CrossRefGoogle Scholar
Dubey, J. P., Rosenthal, B. M. and Speer, C. A. (2001). Sarcocystis lindsayi n. sp. (Protozoa: Sarcocystidae) from the South American opossum, Didelphis albiventris from Brazil. The Journal of Eucaryotic Microbiology 48, 595–603. doi: 10.1111/j.1550-7408.2001.tb00196.xCrossRefGoogle Scholar
Dubey, J. P., Speer, C. A., Fayer, R. (1989). Sarcocystosis of Animals and Man. CRC Press, Boca Raton, FL, USA.Google Scholar
Edgar, R. C. (2004). MUSCLE: a multiple sequence alignment method with reduced time and space complexity. BMC Bioinformatics 19, 113. doi:10.1186/1471-2105-5-113CrossRefGoogle Scholar
Grikienienė, J. and Iezhova, T. (1998). The prevalence of Sarcocystis in some wild birds and poultry in Lithuania and in neighbouring territories. Bulletin of the Scandinavian Society for Parasitology 8, 61–62.Google Scholar
Holmdahl, O. J., Morrision, D. A., Ellis, J. T. and Huong, L. T. (1999). Evolution of ruminant Sarcocystis (Sporozoa) parasites based on small subunit rDNA sequences. Molecular Phylogenetics and Evolution 11, 27–37. doi: 10.1006/mpev.1998.0556.CrossRefGoogle ScholarPubMed
Kalyakin, V. N. and Zasukhin, D. N. (1975). Distribution of Sarcocystis (Protozoa: Sporozoa) in vertebrates. Folia Parasitologica 22, 289–307.Google ScholarPubMed
Kutkienė, L., Sruoga, A. and Butkauskas, D. (2006). Sarcocystis sp. from white-fronted goose (Anser albiforns): cyst morphology and life cycle studies. Parasitology Research 99, 562–565. doi: 10.1007/s00436-007-0813-3.CrossRefGoogle ScholarPubMed
Kutkienė, L., Sruoga, A. and Butkauskas, D. (2008). Sarcocystis sp. from the goldeneye (Bucephala clangula) and the mallard (Anas platyrhynchos): cyst morphology and ribosomal DNA analysis. Parasitology Research 102, 691–696. doi: 10.1007/s00436-007-0813-3.CrossRefGoogle ScholarPubMed
Kutkienė, L., Prakas, P., Sruoga, A. and Butkauskas, D. (2009). Sarcocystis in the birds family Corvidae with description of Sarcocystis cornixi sp. nov. from the hooded crow (Corvus cornix). Parasitology Research 104, 329–336. doi: 10.1007/s00436-008-1196-9.CrossRefGoogle ScholarPubMed
Kutkienė, L., Prakas, P., Sruoga, A. and Butkauskas, D. (2010). The mallard duck (Anas platyrhynchos) as intermediate host for Sarcocystis wobeseri sp. nov. from the barnacle goose (Branta leucopsis). Parasitology Research 107, 879–888. doi: 10.1007/s00436-010-1945-4.CrossRefGoogle ScholarPubMed
Kutkienė, L., Prakas, P., Sruoga, A. and Butkauskas, D. (2012). Description of Sarcocystis anasi sp. nov. and Sarcocystis albifronsi sp. nov. in birds of the oder Anseriformes. Parasitology Research 110, 1043–1046. doi: 10.1007/s00436-011-2588-9.CrossRefGoogle Scholar
Levine, N. D. (1986). The taxonomy of Sarcocystis (Protozoa, Apicomplexa) species. Journal of Parasitology 72, 372–382. doi: 10.2307/3281676.CrossRefGoogle ScholarPubMed
Levine, N. D. and Tadros, W. (1980). Named species and hosts of Sarcocystis (Protozoa: Apicomplexa: Sarcocystidae). Systematic Parasitology 2, 41–59. doi: 10.1007/BF00015094CrossRefGoogle Scholar
Logminas, V. (1990) Fauna of Lithuania. Mokslas, Vilnius, Lithuania.Google Scholar
Morrison, D. A., Bornstein, S., Thebo, P., Wernery, U., Kinne, J. and Mattsson, J. G. (2004). The current status of the small subunit rRNA phylogeny of the coccidia (Sporozoa). International Journal for Parasitology 34, 501–514. doi: 10.1016/j.ijpara.2003.11.006.CrossRefGoogle ScholarPubMed
Odening, K. (1998). The present state of species-systematics in Sarcocystis Lankester, 1882 (Protista, Sporozoa, Coccidia). Systematic Parasitology 41, 209–233. doi: 10.1023/A:1006090232343CrossRefGoogle Scholar
Olias, P., Gruber, A. D., Hafez, H. M., Heydorn, A. O., Mehlhorn, H. and Lierz, M. (2010 a). Sarcocystis calchasi sp. nov. of the domestic pigeon (Columba livia f. domestica) and the Northern goshawk (Accipiter gentilis): light and electron microscopical characteristics. Parasitology Research 106, 577–585. doi: 10.1007/s00436-009-1701-9.CrossRefGoogle ScholarPubMed
Olias, P. P., Olias, L., Krücken, J., Lierz, M. and Gruber, A. D. (2011). High prevalence of Sarcocystis calchasi sporocysts in European Accipiter hawks. Veterinary Parasitology 175, 230–236. doi:10.1016/j.vetpar.2010.10.025.CrossRefGoogle ScholarPubMed
Olias, P., Olias, L., Lierz, M., Mehlhorn, H. and Gruber, A. D. (2010 b). Sarcocystis calchasi is distinct to Sarcocystis columbae sp. nov. from the wood pigeon (Columba palumbus) and Sarcocystis sp. from the sparrowhawk (Accipiter nisus). Veterinay Parasitology 171, 7–14. doi:10.1016/j.vetpar.2010.03.021.CrossRefGoogle ScholarPubMed
Pak, S. M. and Eshtokina, N. V. (1984). Sarcosporidians of birds. In Sarcosporidians of Animals in Kazakhstan (ed. Panin, V. J.), pp. 150–168. Nauka, Alma-Ata, Kazakhstan.Google Scholar
Pinayeva, L. M., Pak, C. M. and Kokhno, L. I. (1998). Sarcocystis of the wild birds of Kazakhstan. Parasitology International 47, (Suppl.) 143.CrossRefGoogle Scholar
Ronquist, F. and Huelsenbeck, J. P. (2003). MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19, 1572–1574. doi: 10.1093/bioinformatics/btg180.CrossRefGoogle ScholarPubMed
Šlapeta, J. R., Modrý, D., Votýpka, J., Jirků, M., Lukeš, J. and Koudela, B. (2003). Evolutionary relationships among cyst-forming coccidia Sarcocystis spp. (Alveolata: Apicomplexa: Coccidea) in endemic African tree vipers and perspective for evolution of heteroxenous life cycle. Molecular Phylogenetics and Evolution 27, 464–475. doi:10.1016/S1055-7903(03)00018-6.CrossRefGoogle ScholarPubMed
Stratford, J. (1999). Ornithological fauna of Baltoji Vokė, an important bird area. Acta Zoologica Lituanica 9, 24–79.CrossRefGoogle Scholar
Svobodova, M. (1996). A Sarcocystis species from goshawk (Accipiter gentilis) with great tit (Parus major) as intermediate host. Acta Protozoologica 35, 223–226.Google Scholar
Tanhauser, S. M., Yowell, C. A., Cutler, T. J., Greiner, E. C., Mackay, R. J. and Dame, J. B. (1999). Multiple DNA markers differentiate Sarcocystis neurona and Sarcocystis falcatula. Journal of Parasitology 85, 221–228. doi: 10.2307/3285623CrossRefGoogle ScholarPubMed
Wenyon, C. M. (1926). Protozoology. A Manual for Medical Men, Veterinarians and Zoologists. Tindall & Cox, Baillitre, London, UK.CrossRefGoogle Scholar
Yabsley, M. J., Ellis, A. E., Stallknecht, D. E. and Howerth, E. W. (2009). Characterization of Sarcocystis from four species of hawks from Georgia, USA. Journal of Parasitology 95, 256–259. doi: 10.1645/GE-1567.1.CrossRefGoogle ScholarPubMed
Žalakevičius, M. (2007). Climate-induced alterations in the Lithuanian migratory avifauna: regional and species-specific aspects. Ekologija 1, 1–9.Google Scholar