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Delta-aminolevulinate dehydratase activity in red blood cells of rats infected with Trypanosoma evansi

Published online by Cambridge University Press:  22 August 2011

RAQUELI T. FRANÇA ,
ALEKSANDRO S. DA SILVA ,
PATRÍCIA WOLKMER ,
VITOR A. OLIVEIRA ,
MARIA E. PEREIRA ,
MARTA L. R. LEAL ,
CÁSSIA B. SILVA ,
MATHEUS A. G. NUNES ,
VALDERI L. DRESSLER  and
CINTHIA M. MAZZANTI
...Show all authors
RAQUELI T. FRANÇA*
Affiliation:
Department of Small Animal, Federal University of Santa Maria, Brazil
ALEKSANDRO S. DA SILVA
Affiliation:
Department of Microbiology and Parasitology, Federal University of Santa Maria, Brazil
PATRÍCIA WOLKMER
Affiliation:
Department of Small Animal, Federal University of Santa Maria, Brazil
VITOR A. OLIVEIRA
Affiliation:
Department of Chemistry, Federal University of Santa Maria, Brazil
MARIA E. PEREIRA
Affiliation:
Department of Chemistry, Federal University of Santa Maria, Brazil
MARTA L. R. LEAL
Affiliation:
Department of Large Animals, Federal University of Santa Maria, Brazil
CÁSSIA B. SILVA
Affiliation:
Department of Small Animal, Federal University of Santa Maria, Brazil
MATHEUS A. G. NUNES
Affiliation:
Department of Chemistry, Federal University of Santa Maria, Brazil
VALDERI L. DRESSLER
Affiliation:
Department of Chemistry, Federal University of Santa Maria, Brazil
CINTHIA M. MAZZANTI
Affiliation:
Department of Small Animal, Federal University of Santa Maria, Brazil
SILVIA G. MONTEIRO
Affiliation:
Department of Microbiology and Parasitology, Federal University of Santa Maria, Brazil
SONIA T. A. LOPES
Affiliation:
Department of Small Animal, Federal University of Santa Maria, Brazil
*
*Corresponding author: Departamento de Pequenos Animais, Universidade Federal de Santa Maria, Camobi – 97, Hospital Veterinário, Sala 103. 97105-900, Santa Maria – RS, Brasil. Tel: and Fax: +55 55 32208814. E-mail: [email protected]
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Summary

The aim of this study was to evaluate the activity of delta-aminolevulinate dehydratase (δ-ALA-D) in red blood cells of rats infected with Trypanosoma evansi and establish its association with haematocrit, serum levels of iron and zinc and lipid peroxidation. Thirty-six male rats (Wistar) were divided into 2 groups with 18 animals each. Group A was non-infected while Group B was intraperitoneally infected, receiving 7·5×106 trypomastigotes per animal. Each group was divided into 3 subgroups of 6 rats and blood was collected during different periods post-infection (p.i.) as follows: day 5 (A1 and B1), day 15 (A2 and B2) and day 30 PI (A3 and B3). Blood samples were collected by cardiac puncture to estimate red blood cell parameters (RBC), δ-ALA-D activity and serum levels of iron, zinc and thiobarbituric acid reactive substances (TBARS). Rats in group B showed a significant (P<0·05) reduction of RBC count, haemoglobin concentration and haematocrit at days 5 and 15 p.i. The activity of δ-ALA-D in blood was significantly (P<0·001) increased at days 15 and 30 p.i. δ-ALA-D activity in blood had a significant (P<0·05) negative correlation with haematocrit (r=−0·61) and haemoglobin (r=−0·70) at day 15 p.i. There was a significant (P<0·05) decrease in serum iron and zinc levels and an increase in TBARS levels (P<0·05) during infection. The δ-ALA-D activity in blood was negatively correlated with the levels of iron (r=−0·68) and zinc (r=−0·57) on day 30 p.i. It was concluded that the increased activity of δ-ALA-D in blood might have occurred in response to the anaemia in remission as heme synthesis was enhanced.

Keywords

Trypanosoma evansidelta-aminolevulinate dehydrataseratsanaemia
Type
Research Article
Information
Parasitology , Volume 138 , Issue 10 , September 2011 , pp. 1272 - 1277
Copyright
Copyright © Cambridge University Press 2011

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