Hostname: page-component-745bb68f8f-cphqk Total loading time: 0 Render date: 2025-01-24T05:55:07.555Z Has data issue: false hasContentIssue false
  • English
  • Français

Cytochemical localization of cytochrome oxidase in tissues of parasitic nematodes

Published online by Cambridge University Press:  06 April 2009

M. Fry  and
J. E. Beesley
M. Fry
Affiliation:
Department of Biochemical Parasitology and Wellcome Research Laboratories, Langley Court, Beckenham, Kent BR3 3BS
J. E. Beesley
Affiliation:
Microscope Unit, Wellcome Research Laboratories, Langley Court, Beckenham, Kent BR3 3BS
Get access Rights & Permissions [Opens in a new window]

Extract

Cytochemical staining with diaminobenzidine (DAB) has been used to locate cytochrome oxidase activity in mitochondria from major tissues of three intestinal parasitic nematodes, Nippostrongylus brasiliensis, Haemonchus contortus and Ascaridia galli. Deposition of DAB reaction product was uniform and relatively intense in mitochondria from all tissues studied in N. brasiliensis. However, mitochondria from different tissues of H. contortus and A. galli varied considerably in their cytochemical staining, decreasing in the order hypodermis < muscle < gut < reproductive tissue. Mitochondria in gut and reproductive tissue of A. galli possessed little or no cytochrome oxidase activity. These results are compatible with respect to oxygen availability and the extent and localization of the mammalian-type aerobic mitochondrial electron transport pathway in intestinal parasitic nematodes.

Type
Research Article
Information
Parasitology , Volume 90 , Issue 1 , February 1985 , pp. 145 - 156
Copyright
Copyright © Cambridge University Press 1985

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Atkinson, H. J. (1980). Respiration in nematodes. In Nematodes as Biological Models, vol. 2 (ed. Zuckerman, B. M.), pp. 101–42. New York and London: Academic Press.Google Scholar
Barrett, J. (1981). Biochemistry of Parasitic Helminths, pp. 72–148. London: MacMillan Publishers Limited.CrossRefGoogle Scholar
Bryant, C. (1970). Electron transport in parasitic helminths and protozoa. Advances in Parasitology 8, 139–72.CrossRefGoogle ScholarPubMed
Fry, M., Bazil, C. & Jenkins, D. C. (1983). A comparison of mitochondrial electron transport in the intestinal parasitic nematodes Nippostrongylus brasiliensis and Ascaridia galli. Comparative Biochemistry and Physiology 75B, 451–60.Google Scholar
Fry, M. & Brazeley, E. P. (1984). NADH-fumarate reductase and succinate dehydrogenase activities in mitochondria of Ascaridia galli and Nippostrongylus brasiliensis. Comparative Biochemistry and Physiology 77B, 143–50.Google Scholar
Fry, M. & Jenkins, D. C. (1983). Nippostrongylus brasiliensis and Ascaridia galli: Mitochondrial respiration in free-living and parasitic stages. Experimental Parasitology 56, 101–6.CrossRefGoogle ScholarPubMed
Fry, M. & Jenkins, D. C. (1984 a). Nematoda: Aerobic respiratory pathways of adult parasitic species. Experimental Parasitology 57, 86–92.Google Scholar
Fry, M. & Jenkins, D. C. (1984 b). Nippostrongylus brasiliensis: the effect of mitochondrial inhibitors on life-cycle stages. Parasitology 88, 163–77.CrossRefGoogle ScholarPubMed
Lloyd, D., Mellor, H. & Williams, J. L. (1983). Oxygen affinity of the respiratory chain of Acanthamoeba castellanii. The Biochemical Journal 214, 47–51.CrossRefGoogle ScholarPubMed
Rodrick, G. E., Long, S. D., Sodeman, W. A. Jr. & Smith, D. L. (1982). Ascaris suum: Oxidative phosphorylation in mitochondria from developing eggs and adult muscle. Experimental Parasitology 54, 235–42.CrossRefGoogle ScholarPubMed
Seligman, A. M., Karnovsky, M. J., Wasserkrug, H. L. & Hanker, J. S. (1968). Nondroplet ultrastructural demonstration of cytochrome oxidase activity with a polymerising osmiophilic reagent, diaminobenzidine (DAB). Journal of Cell Biology 38, 1–14.Google Scholar