Hostname: page-component-586b7cd67f-l7hp2 Total loading time: 0 Render date: 2024-11-27T15:40:29.589Z Has data issue: false hasContentIssue false
  • English
  • Français

Cross-immunity to Schistosoma mansoni and S. haematobium in the hamster

Published online by Cambridge University Press:  06 April 2009

M. A. Smith ,
J. A. Clegg  and
G. Webbe
M. A. Smith
Affiliation:
London School of Hygiene and Tropical Medicine, Winches Farm Field Station, St Albans, Herts, and National Institute for Medical Research, Mill Hill, NW7 1AA
J. A. Clegg
Affiliation:
London School of Hygiene and Tropical Medicine, Winches Farm Field Station, St Albans, Herts, and National Institute for Medical Research, Mill Hill, NW7 1AA
G. Webbe
Affiliation:
London School of Hygiene and Tropical Medicine, Winches Farm Field Station, St Albans, Herts, and National Institute for Medical Research, Mill Hill, NW7 1AA
Get access Rights & Permissions [Opens in a new window]

Summary

Hamsters (WO strain) with a primary infection of Schistosoma mansoni or S. haematobium rapidly developed immunity to homologous challenge judged by the lung recovery assay. Immunity was detected at 4–5 weeks and reached a plateau 6 weeks after infection.

Using this information, hamsters with an 8-week primary infection with S. mansoni or S. haematobium were tested for resistance to homologous reinfection and resistance to a challenge with the other species of schistosome. Primary infection with S. mansoni or S. haematobium conferred a high level of immunity to reinfection with either species of schistosome judged by the perfusion assay, involving recovery of adult worms 6–10 weeks following challenge. Estimation of the level of immunity with the lung recovery assay, 5 days after challenge, indicated that immunity due to a primary infection with S.mansoni acted at or before migration of the challenge through the lungs but immunity stimulated by a primary S. haematobium infection was only partially effective at the lung stage and substantial destruction of challenging organisms occurred at a later stage of development.

Antibodies in immune sera of hamsters with a primary S. mansoni or S. haematobium infection were shown to bind to common antigens on the surface of young schistosomula of either species by u.v. microscopy using as detecting agent a fluorescein-labelled rabbit antiserum directed against hamster globulins.

Type
Research Article
Information
Parasitology , Volume 73 , Issue 1 , August 1976 , pp. 53 - 64
Copyright
Copyright © Cambridge University Press 1976

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Butterworth, A. E., Sturrock, R. F., Houba, V. & Rees, V. M. (1974). Antibody dependent cell-mediated damage to schistosomula in vitro. Nature, London 252, 503–5.CrossRefGoogle ScholarPubMed
Capron, A., Bazin, H., Dessaint, J. & Capron, M. (1975). Involvement of specific IgE antibodies in the immune adherence of normal macrophages to Schistosoma mansoni schistosomules. Nature, London 253, 474–5.CrossRefGoogle Scholar
Capron, A., Capron, M., Dupas, H., Bout, D. & Petitprez, A. (1974). Étude in vitro des phénomènes immunologiques dans la schistosomiase humaine et expérimentale – I. Étude comparative in vitro de l'activité léthale d'immunsérums sur les formes immatures et sur les adultes de Schistosoma mansoni. International Journal for Parasitology 4, 613–23.CrossRefGoogle Scholar
Capron, A., Vernes, A., Biguet, J. & Rose, F. (1966). Les précipitines sériques dans les bilharzioses humaines et expérimentales à Schistosoma mansoni, S. haematobium et S.japonicum. Annales de Parasitologie, Humaine et Comparée 41, 123–87.Google ScholarPubMed
Clarke, V de V. (1966). The influence of acquired resistance in the epidemiology of bilharziasis. Central African Journal of Medicine 12 (b), Supplement 1–30.Google ScholarPubMed
Clegg, J. A. & Smithebs, S. R. (1972). The effects of immune rhesus monkey serum on schistosomula of Schistosoma mansoni during cultivation in vitro. International Journal for Parasitology 2, 79–98.CrossRefGoogle ScholarPubMed
Cohen, A. & Schlesinger, M. (1970). Absorption of guinea-pig serum with agar. A method for elimination of its cytoxicity for murine thymus cells. Transplantation 10, 130–2.CrossRefGoogle Scholar
Dean, D. A., Wistar, R. & Chen, P. (1975). Immune response of guinea-pigs to Schistosoma mansoni. I. In vitro effects of antibody and neutrophils, eosinophils and macrophages on schistosomula. American Journal of Tropical Medicine and Hygiene 24, 74–82.CrossRefGoogle ScholarPubMed
Dean, D. A., Wistar, R. & Murrell, K. D. (1974). Combined in vitro effects of rat antibody and neutrophilic leucocytes on schistosomula of Schistosoma mansoni. American Journal of Tropical Medicine and Hygiene 23, 420–8.CrossRefGoogle ScholarPubMed
Goldman, M. (1968). In Fluorescent Antibody Methods, pp. 101–2. London and New York: Academic Press.Google Scholar
Mahmoud, A. A. F., Warren, K. S. & Peters, P. A. (1975). The rôle of eosinophils in acquired resistance to Schistosoma mansoni infection in mice. In International Conference on Schistosomiasis, Cairo, Egypt,October 1975, pp. 179–81.Google Scholar
Massoud, J. & Nelson, G. S. (1972). Studies on heterologous immunity in schistosomiasis. 6. Observations on cross-immunity to Omithobilharzia turkestanieum, Schistosoma bovis, S. mansoni and S. haematobium in mice, sheep and cattle in Iran. Bulletin of the World Health Organization 47, 591–600.Google Scholar
McLaren, D. J., Clegg, J. A. & Smithers, S. R. (1975). Acquisition of host antigens by young Schistosoma mansoni in mice: correlation with failure to bind antibody in vitro. Parasitology 70, 67–75.CrossRefGoogle ScholarPubMed
Moore, D. V. & Meleney, H. E. (1954). Comparative susceptibility of common laboratory animals to experimental infection with Schistosoma haematobium. Journal of Parasitology 40, 392–7.CrossRefGoogle ScholarPubMed
Murrell, K. D., Dean, D. A. & Stafford, E. E. (1975). Resistance to infection with Schistosoma mansoni after immunization with worm extracts or live cercariae: rôle of cytotoxic antibody in mice and guinea-pigs. American Journal of Tropical Medicine and Hygiene 24, 955–62.CrossRefGoogle ScholarPubMed
Nelson, G. S., Amin, M. A., Saoud, M. F. A. & Teesdale, C. (1968). Studies on heterologous immunity in schistosomiasis. I. Heterologous schistosome immunity in mice. Bulletin of the World Health Organization 38, 9–17.Google ScholarPubMed
Perez, H. A. (1974). Investigation on the mechanism of protective immunity to Schistosoma mansoni in the laboratory rat. Ph.D. Thesis, Brunei University, Uxbridge.Google Scholar
Perez, H. A., Clegg, J. A. & Smithers, S. R. (1974). Acquired immunity to Schistosoma mansoni in the rat: measurement of immunity by the lung recovery assay. Parasitology 69, 349–59.CrossRefGoogle Scholar
Preston, J. M., Nelson, G. S. & Saeed, A. A. (1972). Studies on heterologous schistosoma immunity in sheep. Bulletin of the World Health Organization 47, 587–90.Google ScholarPubMed
Purnell, R. E. (1966). Acquired resistance in hamsters to infection with Schistosoma haematobium. Transactions of the Royal Society of Tropical Medicine and Hygiene 60, 463–6.CrossRefGoogle ScholarPubMed
Sher, A., Mackenzie, P. & Smithers, S. R. (1974). Decreased recovery of invading parasites from the lungs as a parameter of acquired immunity to schistosomiasis in the mouse. The Journal of Infectious Diseases 130, 626–33.CrossRefGoogle ScholarPubMed
Sher, A., Smithers, S. R. & Mackenzie, P. (1975). Passive transfer of acquired resistance to Schistosoma mansoni in laboratory mice. Parasitology 70, 347–57.CrossRefGoogle ScholarPubMed
Sher, A., Kusel, J. R., Perez, H. A. & Clegg, J. A. (1974). Partial isolation of a membrane antigen which induces the formation of antibodies lethal to schistosomes cultured in vitro. Clinical Experimental Parasitology 18, 357–69.Google ScholarPubMed
Smith, M. A. & Clegg, J. A. (1976). Different levels of acquired immunity to Schistosoma mansoni in two strains of hamster. Parasitology 73, 47–52.CrossRefGoogle ScholarPubMed
Smith, M. A. & Webbe, G. (1974). Damage to schistosomula of Schistosoma haematobium in vitro by immune baboon and human sera and absence of cross-reaction with Schistosoma mansoni. Transactions of the Royal Society of Tropical Medicine and Hygiene 68, 70–1.CrossRefGoogle ScholarPubMed
Smith, M. A., Clegg, J. A. & Webbe, G. (1976). Culture of Schistosoma haematobium in vivo and in vitro. Annals of Tropical Medicine and Parasitology 70, 101–7.CrossRefGoogle ScholarPubMed
Smith, M. A., Clegg, J. A., Kusel, J. R. & Webbe, G. (1975). Lung inflammation in immunity to Schistosoma mansoni. Experientia 31, 595–6.CrossRefGoogle ScholarPubMed
Smithers, S. R. & Terry, R. J. (1965). The infection of laboratory hosts with cercariae of Schistosoma mansoni and the recovery of adult worms. Parasitology 55, 695–700.CrossRefGoogle ScholarPubMed
Wright, C. A. & Bennett, M. S. (1967). Studies on S. haematobium in the laboratory. I. A strain from Durban, South Africa. Transactions of the Royal Society of Tropical Medicine and Hygiene 61, 221–7.CrossRefGoogle Scholar