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Corilagin ameliorates schistosomiasis hepatic fibrosis through regulating IL-13 associated signal pathway in vitro and in vivo

Published online by Cambridge University Press:  21 July 2016

HUA-RONG LI ,
GANG LI ,
MAN LI ,
SHU-LING ZHANG ,
HENG WANG ,
TAO LUO ,
FEI WU ,
JI-HUA DONG ,
YUAN-JIN GUO  and
LEI ZHAO
HUA-RONG LI
Affiliation:
Department of Infectious Diseases, Institute of Infection and Immunology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, P.R. China Department of Integrated Traditional Chinese and Western Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, P.R. China
GANG LI
Affiliation:
Department of Infectious Diseases, Renmin Hospital, Hubei University of Medicine, Shiyan 442000, P.R. China
MAN LI
Affiliation:
Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, P.R. China
SHU-LING ZHANG*
Affiliation:
Department of Infectious Diseases, Institute of Infection and Immunology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, P.R. China
HENG WANG
Affiliation:
Department of Neurosurgery, The 2nd Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, P.R. China
TAO LUO
Affiliation:
Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, P.R. China
FEI WU
Affiliation:
Department of Neurology, Wuhan General Hospital of Guangzhou Military Command, Wuhan 430070, P.R. China
JI-HUA DONG
Affiliation:
Central Lab, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, P.R. China
YUAN-JIN GUO
Affiliation:
Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, P.R. China
LEI ZHAO*
Affiliation:
Department of Infectious Diseases, Institute of Infection and Immunology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, P.R. China
*
*Corresponding authors: Department of Infectious Diseases, Institute of Infection and Immunology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, P.R. China. Tel: +86-27-85726783. Fax: +86-27-87716917. E-mail: [email protected]
*Corresponding authors: Department of Infectious Diseases, Institute of Infection and Immunology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, P.R. China. Tel: +86-27-85726783. Fax: +86-27-87716917. E-mail: [email protected]
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Summary

Interleukin (IL)-13-associated signal pathway plays an important role in schistosomiasis hepatic fibrosis. In this study we tried to investigate the effects of corilagin to ameliorate schistosomiasis hepatic fibrosis through regulating IL-13-associated signal pathway in vitro and in vivo. Cellular model was set up with hepatic stellate cells-T6 cells stimulated by rIL-13 and male Balb/c mice were infected with Schistosoma japonicum cercariaeas as animal model. Liver histological changes were observed with haematoxylin and eosin staining. Masson staining was employed to observe the change of egg granulomas. Expression of Col (collagen) and Col III were examined with Immunohistochemistry. Western bolt was employed to detect the JAK-1 and IL13Rα1 proteins. The mRNA expression of Col I, Col III, IL-13, JAK-1 and IL13Rα1 were tested by quantitative polymerase chain reaction. As a result, less inflammatory changes were found in all corilagin groups compared with model group and praziquantel group. The mRNA levels of Col I, Col III, IL-13, JAK-1 and IL13Rα1 were significantly decreased after corilagin intervention (P < 0·01). JAK-1 and IL-13Rα1 protein levels were also greatly decreased in the corilagin groups (P < 0·01). In conclusion, corilagin could ameliorate schistosomiasis hepatic fibrosis by down-regulating the expression of IL-13 and signal molecules in IL-13 pathway.

Keywords

corilaginIL-13; schistosomiasisliver fibrosis
Type
Research Article
Information
Parasitology , Volume 143 , Issue 12 , October 2016 , pp. 1629 - 1638
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Copyright
Copyright © Cambridge University Press 2016 

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