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Coproantigen detection in rats experimentally infected with Strongyloides ratti

Published online by Cambridge University Press:  06 April 2009

C. Nageswaran ,
P. S. Craig  and
E. Devaney
C. Nageswaran
Affiliation:
Department of Parasitology, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA
P. S. Craig
Affiliation:
Department of Biological Sciences, University of Salford, Salford M5 4WT
E. Devaney
Affiliation:
Department of Veterinary Parasitology, University of Glasgow, Glasgow G61 1QH
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Summary

The possibility of coproantigen detection in the diagnosis of strongyloidiasis was investigated in rats experimentally infected with Strongyloides ratti. A capture ELISA was developed using affinity-purified polyclonal antibodies raised in rabbits against adult and filariform larval somatic antigens. The first detection of coproantigen coincided with patency and the coproantigen level remained elevated even when the parasite count was low. Following challenge infection, when examination of stool for larvae or ova was negative, coproantigen was detected by anti-adult somatic capture ELISA. Despite the use of antisera raised against crude worm extracts, the test was specific showing only low cross-reactivity with Necator americanus and Syphacia muris.

Keywords

Strongyloides ratticoproantigen detectionhumoral responseexperimental infection
Type
Research Article
Information
Parasitology , Volume 108 , Issue 3 , April 1994 , pp. 335 - 342
Copyright
Copyright © Cambridge University Press 1994

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References

REFERENCES

Allan, J. C., Avila, G., Garcia Noval, J., Flisser, A. & Craig, P. S. (1990). Immunodiagnosis of taeniasis by coproantigen detection. Parasitology 101, 473–7.CrossRefGoogle ScholarPubMed
Allan, J. C. & Craig, P. S. (1989). Coproantigens in gut tapeworm infections: Hymenolepis diminuta in rats. Parasitology Research 76, 68–73.CrossRefGoogle ScholarPubMed
Allan, J. C., Craig, P. S., Garcia Noval, J., Mencos, F., Liu, D., Wang, Y., Wen, H., Zhou, P., Stringer, R., Rogan, M. & Zeyhle, E. (1992). Coproantigen detection for immunodiagnosis of echinococcosis and taeniasis in dogs and humans. Parasitology 104, 347–55.CrossRefGoogle ScholarPubMed
Ashford, R. W., Hall, A. J. & Babona, D. (1981). Distribution and abundance of intestinal helminths in man in western Papua New Guinea with special reference to Strongyloides. Annals of Tropical Medicine and Parasitology 75, 269–79.CrossRefGoogle ScholarPubMed
Ashford, R. W., Vince, J. D., Gratten, M. J. & Miles, W. E. (1978). Strongyloides infection associated with acute infantile disease in Papua New Guinea. Transactions of the Royal Society of Tropical Medicine and Hygiene 72, 554.CrossRefGoogle ScholarPubMed
Bailey, J. W. (1989). A serological test for the diagnosis of Strongyloides antibodies in ex Far East Prisoners of War. Annals of Tropical Medicine and Parasitology 83, 241–7.CrossRefGoogle ScholarPubMed
Bradford, M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein-dye binding. Analytical Biochemistry 72, 248–54.CrossRefGoogle ScholarPubMed
Bradley, S. L., Dines, D. E. & Brewer, N. S. (1978). Disseminated Strongyloides stercoralis in an immunosuppressed host. Mayo Clinic Proceedings 53, 332–5.Google Scholar
Carroll, S. M., Karthigasu, K. T. & Grove, D. I. (1981). Serodiagnosis of human strongyloidiasis by an enzyme-linked immunosorbent assay. Transactions of the Royal Society of Tropical Medicine and Hygiene 75, 706–8.CrossRefGoogle ScholarPubMed
Craft, J. C. & Nelson, J. D. (1982). Diagnosis of giardiasis by counterimmunoelectrophoresis of feces. Journal of Infectious Diseases 145, 499–504.CrossRefGoogle ScholarPubMed
De Vault, G. A. Jr, King, J. W., Rohr, M. S., Landreneau, M. D., Brown, S. T. III & McDonald, J. C. (1990). Opportunistic infections with Strongyloides stercoralis in renal transplantation. Reviews of Infectious Diseases 12, 653–71.CrossRefGoogle ScholarPubMed
Genta, R. M. & Well, G. J. (1982). Antibodies to Strongyloides stercoralis larval surface antigens in chronic strongyloidiasis. Laboratory investigation 47, 87–90.Google ScholarPubMed
Green, E. L. (1986). Immunological detection of parasite antigen in faeces. Parasitology Today 2, 198–9.CrossRefGoogle ScholarPubMed
Grundy, M. S., Voller, A. & Warhurst, D. (1987). An enzyme linked immunosorbent assay for the detection of Entamoeba histolytica antigens in faecal material. Transactions of the Royal Society of Tropical Medicine and Hygiene 86, 627–32.CrossRefGoogle Scholar
Higenbottam, T. W. & Heard, B. E. (1976). Opportunistic pulmonary strongyloidiasis complicating asthma treated with steroids. Thorax 31, 226–33.CrossRefGoogle ScholarPubMed
Klein, R. A., Cleri, D. J., Dode, V. & Barasilus, T. A. (1983). Disseminated Strongyloides stercoralis: a fatal case eluding diagnosis. Southern Medical Journal 76, 1438–40.CrossRefGoogle ScholarPubMed
McKerrow, J. H., Brindley, P., Brown, M., Gam, A. A., Staunton, C. & Neva, F. A. (1990). Strongyloides stercoralis: identification of a protease that facilitates penetration of skin by the infective larvae. Experimental Parasitology 70, 134–43.CrossRefGoogle Scholar
Moqbel, R. & Denham, D. A. (1977). Strongyloides ratti. 1. Parasitological observations on primary and secondary infections in the small intestine of rats. Journal of Helminthology 51, 301–8.CrossRefGoogle ScholarPubMed
Murrell, K. D. (1980). Strongyloides ratti: acquired resistance in the rat to the preintestinal migrating larvae. Experimental Parasitology 50, 417–25.CrossRefGoogle Scholar
Neva, F. A., Gam, A. A. & Burke, J. (1981). Comparison of larval antigens in an enzyme-linked immunosorbent assay for strongyloidiasis in humans. Journal of Infectious Diseases 144, 427–32.CrossRefGoogle Scholar
Northern, C. & Grove, D. I. (1987). Antigenic analysis of Strongyloides ratti infective larvae and adult worms. Immunology and Cell Biology 65, 231–9.CrossRefGoogle ScholarPubMed
Sirisinha, S., Chawengkirttikul, R., Amornpant, S., Mongkolsuk, S. & Panyim, S. (1991). Detection of Opisthorchis viverrini by monoclonal antibody based ELISA and DNA hybridization. American Journal of Tropical Medicine and Hygiene 44, 140–5.CrossRefGoogle ScholarPubMed
Thompson, J. R. & Berger, R. (1991). Fatal adult respiratory distress syndrome following successful treatment of pulmonary strongyloidiasis. Chest 99, 772–4.CrossRefGoogle ScholarPubMed
Viscidi, R., Laughon, B. E., Hanvanich, M., Bartlett, J. G. & Yolken, R. H. (1984). Improved enzyme immunoassays for the detection of antigens in fecal specimens. Investigation and correction of interfering factors. Journal of Immunological Methods 67, 129–43.CrossRefGoogle ScholarPubMed
Wilson, M. B. & Nakane, P. K. (1978). Recent developments in the periodate method of conjugating horseradish peroxidase (HRPO) to antibodies. In Immunofluorescence and Related Staining Techniques (ed. Knapp, W., Holubar, K. & Wick, G.), pp. 215–24. Elsevier/North-Holland Biomedical Press.Google Scholar
Youssef, F. G., Mansour, N. S. & Aziz, A. G. (1991). Early diagnosis of human fascioliasis by the detection of copro-antigens using counterimmunoelectrophoresis. Transactions of the Royal Society of Tropical Medicine and Hygiene 85, 383–4.CrossRefGoogle ScholarPubMed