Hostname: page-component-78c5997874-4rdpn Total loading time: 0 Render date: 2024-11-14T21:25:06.376Z Has data issue: false hasContentIssue false
  • English
  • Français

Continuous cultivation of intraerythrocytic Plasmodium falciparum in a serum-free medium with the use of a growth-promoting factor

Published online by Cambridge University Press:  06 April 2009

H. Asahi  and
T. Kanazawa
H. Asahi
Affiliation:
Department of Parasitology, National Institute of Health, 23–1, Toyama 1-chome, Shinjuku-ku, Tokyo 162, Japan
T. Kanazawa
Affiliation:
Department of Parasitology, National Institute of Health, 23–1, Toyama 1-chome, Shinjuku-ku, Tokyo 162, Japan
Get access Rights & Permissions [Opens in a new window]

Summary

Serum-free media were used to culture Plasmodium falciparum. A commercial preparation, Daigo's GF21 developed as a growth-promoting factor for many kinds of mammalian cells, and consisting of the 55–70% ammonium sulphate fraction of adult bovine serum, insulin, transferrin, ethanolamine and sodium selenite, was found to sustain growth of the parasite when Daigo's T was employed as a basal medium. The optimal Daigo's GF21 concentration for parasite growth was between 5 and 20% (v/v), with the best results at 10%. Differential counts indicated that Daigo's GF21 is essential for schizogony. Established serum-free medium, GIT, consisting of Daigo's T basal medium and Daigo's GF21, yielded good parasite growth without any supplementation. Growth-promoting factor derived from adult bovine serum in Daigo's GF21 was shown to be crucial to parasite growth. The results presented here will not only be of practical value, but will provide important information about the developmental requirements for the parasite.

Keywords

Plasmnodium falciparumhuman malariaserum-free culturegrowth-promoting factor
Type
Research Article
Information
Parasitology , Volume 109 , Issue 4 , November 1994 , pp. 397 - 401
Copyright
Copyright © Cambridge University Press 1994

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Divo, A. A. & Jensen, J. B. (1982). Studies on serum requirements for the cultivation of Plasmodium falciparum 1. Animal sera. Bulletin of the World Health Organization 60, 565–9.Google ScholarPubMed
Grellier, P., Rigomier, D., Clavey, V., Fruchart, J-C. & Schrevel, J. (1991). Lipid traffic between high density lipoproteins and Plasmodium falciparum-infected red blood cells. Journal of Cell Biology 112, 267–77.CrossRefGoogle ScholarPubMed
Ifediba, T. & Vanderberg, J. P. (1980). Peptones and calf serum as a replacement for human serum in the cultivation of Plasmodium falciparum. Journal of Parasitology 66, 236–9.CrossRefGoogle ScholarPubMed
Jensen, J. B. (1979). Some aspects of serum requirements for continuous cultivation of Plasmodium falciparum. Bulletin of the World Health Organization 57 (Suppl. 1), 27–31.Google ScholarPubMed
Kudo, T., Morishita, R., Suzuki (SENO), H. & Tachibana, T. (1987). A great improvement of fusion efficiency in mouse B cell hybridoma production by use of the new culture medium, GIT. Tohoku Journal of Experimental Medicine 153, 55–66.CrossRefGoogle ScholarPubMed
Lambros, C. & Vanderberg, J. P. (1979). Synchronization of Plasmodium falciparum erythrocytic stages in culture. Journal of Parasitology 65, 418–20.CrossRefGoogle ScholarPubMed
Lingnau, A., Margos, G., Maier, W. A. & Seitz, H. M. (1993). Serum-free cultivation of Plasmodium falciparum gametocytes in vitro. Parasitology Research 79, 378–84.CrossRefGoogle ScholarPubMed
Nivet, C., Guillotte, M. & Pereira Da Silva, L. (1983). Plasmodium falciparum: one-step growth in semi-defined medium and the stimulatory effect of human seric lipoproteins and liposomes. Experimental Parasitology 55, 147–51.CrossRefGoogle ScholarPubMed
Ofulla, A. V. O., Okoye, V. C. N., Khan, B., Githure, J. I., Roberts, C. R., Johnson, A. J. & Martin, S. K. (1993). Cultivation of Plasmodium falciparum parasites in a serum-free medium. American Journal of Tropical Medicine and Hygiene 49, 335–40.CrossRefGoogle Scholar
Sasai, S., Umemoto, S. & Fujimoto, T. (1985). Establishment of a medium for mass culture of mammalian cells. 3rd International Cell Culture Congress Sendai, Japan, p. 46. (Abstract.)Google Scholar
Sax, L. J. & Rieckmann, K. H. (1980). Use of rabbit serum in the cultivation of Plasmodium falciparum. Journal of Parasitology 66, 621–4.CrossRefGoogle ScholarPubMed
Trager, W. & Jensen, J. B. (1976). Human malaria parasites in Continuous culture. Science 193, 673–5.CrossRefGoogle ScholarPubMed
Vernot, J. P. & Wasserman, M. (1990). Plasmodium falciparum: Increased and multiple invasion during short periods of time. Journal of Protozoology 37, 47–9.CrossRefGoogle ScholarPubMed
Willet, G. P. & Canfield, C. J. (1984). Plasmodium falciparum: Continuous cultivation of erythrocyte stages in plasma-free culture medium. Experimental Parasitology 57, 76–80.CrossRefGoogle ScholarPubMed