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Compatibility in the Biomphalaria glabrata/Echinostoma caproni model: new candidate genes evidenced by a suppressive subtractive hybridization approach

Published online by Cambridge University Press:  13 November 2006

A. BOUCHUT ,
C. COUSTAU ,
B. GOURBAL  and
G. MITTA
A. BOUCHUT
Affiliation:
Parasitologie Fonctionnelle et Evolutive, UMR 5555, CNRS Université de Perpignan Perpignan, France
C. COUSTAU
Affiliation:
U547, INSERM Institut Pasteur de Lille, Lille, France
B. GOURBAL
Affiliation:
Parasitologie Fonctionnelle et Evolutive, UMR 5555, CNRS Université de Perpignan Perpignan, France
G. MITTA
Affiliation:
Parasitologie Fonctionnelle et Evolutive, UMR 5555, CNRS Université de Perpignan Perpignan, France
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Abstract

In order to elucidate mechanisms underlying snail/echinostome compatibility, numerous molecular studies comparing transcripts and proteins of Biomphalaria glabrata susceptible or resistant to Echinostoma caproni were undertaken. These studies focused on plasma and haemocytes of the two strains and revealed that some transcripts and/or proteins were differentially expressed between strains. The aim of the present study was to develop a complementary transcriptomic approach by constructing subtractive libraries. This work revealed some candidate transcripts already identified in previous studies (calcium-binding proteins and glycolytic enzymes) as well as novel candidate transcripts that were differentially represented between strains of B. glabrata. Among these newly identified genes, we revealed several genes potentially involved in immune processes encoding proteases, protease inhibitors, a lectin, an aplysianin-like molecule, and cell adhesion molecules.

Keywords

Biomphalaria glabrataEchinostoma capronihost-parasite interactionssuppression subtractive hybridizationcompatibility
Type
Research Article
Information
Parasitology , Volume 134 , Issue 4 , April 2007 , pp. 575 - 588
Copyright
© 2006 Cambridge University Press

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