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Comparison of the proteins in salivary glands, saliva and haemolymph of Rhipicephalus appendiculatus female ticks during feeding

Published online by Cambridge University Press:  06 April 2009

H. Wang  and
P. A. Nuttall
H. Wang
Affiliation:
NERC Institute of Virology and Environmental Microbiology, Mansfield Road, Oxford OX1 3SR
P. A. Nuttall
Affiliation:
NERC Institute of Virology and Environmental Microbiology, Mansfield Road, Oxford OX1 3SR
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Summary

To compare the proteins in salivary glands, saliva and haemolymph of Rhipicephalus appendiculatus female ticks, antisera were prepared from guinea-pigs immunized with soluble denatured salivary gland extracts (SGE). The extracts were derived from R. appendiculatus female ticks that were either unfed (day 0) or partly fed (day 6). The sera were used in immunoblotting, following SDS–polyacrylamide gel electrophoresis, to examine the antigen profiles during the course of tick feeding on guinea-pigs. Day 0 and day 6 SGE antisera appeared to detect common proteins in the different tick samples. For example, haemolymph apparently shared some of the small protein bands (31·5–34 kDa) detected in SGEs. These small proteins appeared in both samples at the same stage of feeding, suggesting that haemolymph and salivary glands not only have common antigens but may also share some functions. Furthermore, a number of protein bands were detected in haemolymph before they were apparent in the salivary glands or saliva. Thus some antigens detected in the salivary glands and saliva may be derived from the haemolymph. The results indicate that the host may be exposed to tick saliva antigens that are also present in the haemolymph. We discuss the significance of these observations with regard to the induction of host immunity to ticks and the development of tick vaccines.

Keywords

Rhipicephalus appendiculatustick proteinsimmunoglobulinimmunoblotting
Type
Research Article
Information
Parasitology , Volume 109 , Issue 4 , November 1994 , pp. 517 - 523
Copyright
Copyright © Cambridge University Press 1994

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References

REFERENCES

Barriga, O. O., Andujar, F. & Andrzejewski, W. J. (1991 a). Manifestations of immunity in sheep repeatedly infested with Amblyomma americanum ticks. Journal of Parasitology 77, 703–9.CrossRefGoogle ScholarPubMed
Barriga, O. O., Andujar, F., Sahibi, H. & Andrzejewski, W. J. (1991 b). Antigens of Amblyomma americanum ticks recognized by repeatedly infested sheep. Journal of Parasitology 77, 710–16.CrossRefGoogle ScholarPubMed
Ben-Yakir, D., Fox, J. C., Homer, J. T. & Barker, R. W. (1986). Quantitative studies of host immunoglobulin G passage into the haemocoel of the ticks Amblyomma americanum and Dermacentor variabilis. In Morphology, Physiology, and Behavioural Biology of Ticks, Ellis Horwood Series in Acarology (ed. Sauer, J. R. & Hair, J. A.), pp. 329–41. Chichester, UK: Ellis Horwood.Google Scholar
Binnington, K. C. & Kemp, D. H. (1980). Role of tick salivary glands in feeding and disease transmission. Advances in Parasitology 18, 315–39.CrossRefGoogle ScholarPubMed
Brown, S. J. (1988). Characterization of tick antigens inducing host immune resistance. II. Description of rabbit acquired immunity to Amblyomma americanum ticks and identification of potential tick antigens by Western blot analysis. Veterinary Parasitology 28, 205–59.CrossRefGoogle ScholarPubMed
Dharampaul, S., Kaufman, W. R. & Belosevic, M. (1992). Differential recognition of saliva antigens from the ixodid tick Amblyomma hebraeum by sera from infested and immunized rabbits. Journal of Medical Entomology 30, 262–6.CrossRefGoogle Scholar
Harris, E. L. V. & Angal, S. (1989). Protein Purification Methods. 1. Protein Purification. Oxford: IRL Press.Google Scholar
Jaworski, D. C., Muller, M. T., Simmen, F. A. & Needham, G. R. (1990). Amblyomma americanum: identification of tick salivary gland antigens from unfed and early feeding females with comparisons to Ixodes dammini and Dermacentor variabilis. Experimental Parasitology 70, 217–26.CrossRefGoogle ScholarPubMed
Jones, L. D., Davies, C. R., Steele, G. M. & Nuttall, P. A. (1988). The rearing and maintenance of ixodid and argasid ticks in the laboratory. Journal of Animal Technology 39, 99–106.Google Scholar
Jones, L. D., Kaufman, W. R. & Nuttall, P. A. (1992). Feeding site modification by tick saliva resulting in enhanced virus transmission. Experientia 48, 779–82.CrossRefGoogle Scholar
Kaufman, W. R. (1978). Actions of drugs and their antagonists on salivary secretion in tick. American Journal of Physiology 235, R76–R81.Google Scholar
Kaufman, W. R. (1989). Tick–host interaction: a synthesis of current concepts. Parasitology Today 5, 47–56.CrossRefGoogle ScholarPubMed
Needham, G. R., Jaworski, D. C., Simmen, F. A., Sherif, N. & Muller, M. T. (1989). Characterization of ixodid tick salivary-gland gene products, using recombinant DNA technology. Experimental and Applied Acarology 7, 21–32.CrossRefGoogle ScholarPubMed
Neitz, A. W. H. & Vermeulen, N. M. J. (1987). Biochemical studies on the salivary glands and haemolymph of Amblyomma hebraeum. Onderstepoort Journal of Veterinary Research 54, 443–50.Google ScholarPubMed
Nuttall, P. A., Jones, L. D., Labuda, M. & Kaufman, W. R. (1994). Adaptations of arboviruses to ticks. Journal of Medical Entomology 31, 1–9.CrossRefGoogle ScholarPubMed
Oaks, J. F., McSwain, J. L., Bantle, J. A., Essenberg, R. C. & Sauer, J. R. (1991). Putative new expression of genes in ixodid tick salivary gland development during feeding. Journal of Parasitology 77, 378–83.CrossRefGoogle ScholarPubMed
Ribeiro, J. M. C. (1987). Role of saliva in blood-feeding by arthropods. Annual Review of Entomology 32, 463–78.CrossRefGoogle ScholarPubMed
Shapiro, S. Z., Buscher, G. & Dobbelaere, D. A. E. (1987). Acquired resistance to Rhipicephalus appendiculatus (Acari: Ixodidae): identification of an antigen eliciting resistance in rabbits. Journal of Medical Entomology 24, 147–54.CrossRefGoogle ScholarPubMed
Shapiro, S. Z., Voigt, W. P. & Ellis, J. A. (1989). Acquired resistance to ixodid ticks induced by tick cement antigen. Experimental and Applied Acarology 7, 33–41.CrossRefGoogle ScholarPubMed
Shapiro, S. Z., Voigt, W. P. & Fujisaki, K. (1986). Tick antigens recognized by serum from a guinea pig tick immune to infestation with the tick Rhipicephalus appendiculatus. Journal of Parasitology 72, 454–63.CrossRefGoogle ScholarPubMed
Sonenshine, D. E. (1991). Biology of Ticks, Vol. 1. London: Oxford University Press.Google Scholar
Towbin, H., Staehelin, T. & Gordon, J. (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, USA 76, 4350–4.CrossRefGoogle ScholarPubMed
Varma, M. G. R., Heller-Haupt, A., Trinder, P. K. E. & Langi, A. O. (1990). Immunization of guinea-pigs against Rhipicephalus appendiculatus adult ticks using homogenates from unfed immature ticks. Immunology 71, 133–8.Google ScholarPubMed
Wang, H. & Nuttall, P. A. (1994). Excretion of host immunoglobulin in tick saliva and detection of IgG-binding proteins in tick haemolymph and salivary glands. Parasitology 109, 525–30.CrossRefGoogle ScholarPubMed
Willadsen, P., Eisemann, C. H. & Tellam, R. L. (1993). ‘Concealed’ antigens: expanding the range of immunological targets. Parasitology Today 9, 132–5.CrossRefGoogle ScholarPubMed