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Comparison of parasitological, immunological and molecular methods for evaluation of fecal samples of immunosuppressed rats experimentally infected with Strongyloides venezuelensis

Published online by Cambridge University Press:  07 October 2015

LEILANE A. CHAVES ,
ANA LÚCIA R. GONÇALVES ,
FABIANA M. PAULA ,
NEIDE. M. SILVA ,
CLÁUDIO V. SILVA ,
JULIA M. COSTA-CRUZ  and
MICHELLE A. R. FREITAS
LEILANE A. CHAVES
Affiliation:
Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia (UFU), Av. Pará 1720, Uberlândia, 38400-902 Minas Gerais, Brazil
ANA LÚCIA R. GONÇALVES
Affiliation:
Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia (UFU), Av. Pará 1720, Uberlândia, 38400-902 Minas Gerais, Brazil
FABIANA M. PAULA
Affiliation:
Laboratório de Investigação Médica, Hospital de Clínicas da Universidade de São Paulo, São Paulo, Brazil
NEIDE. M. SILVA
Affiliation:
Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia (UFU), Av. Pará 1720, Uberlândia, 38400-902 Minas Gerais, Brazil
CLÁUDIO V. SILVA
Affiliation:
Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia (UFU), Av. Pará 1720, Uberlândia, 38400-902 Minas Gerais, Brazil
JULIA M. COSTA-CRUZ
Affiliation:
Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia (UFU), Av. Pará 1720, Uberlândia, 38400-902 Minas Gerais, Brazil
MICHELLE A. R. FREITAS*
Affiliation:
Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia (UFU), Av. Pará 1720, Uberlândia, 38400-902 Minas Gerais, Brazil
*
* Corresponding author: Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Avenida Pará 1720, 38400-902 Uberlândia, Minas Gerais, Brazil. E-mail: [email protected]
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Summary

Definitive diagnosis of strongyloidiasis in humans is typically achieved by detection of larvae in fecal samples. However, limitations on sensitivity of parasitological methods emphasize the need for more robust diagnostic methods. The aim of this study was to compare the diagnostic value of three methods: eggs per gram of feces (EPG), coproantigen detection by enzyme linked immunosorbent assay (ELISA), and DNA detection by conventional polymerase chain reaction (PCR). The assays were performed at 0 and 5, 8, 13, 21 and 39 days post-infection (dpi) using fecal samples from experimentally infected immunocompetent and immunosuppressed rats. In immunocompetent rats, eggs were detected in feces on days 5, 8 and 13 dpi; coproantigen detection and PCR amplification were successful at all post-infection time points (5, 8, 13, 21 and 39 dpi). In immunosuppressed rats, eggs were detected at 5, 8, 13 and 21; coproantigen detection and PCR amplification were successful at all post-infection time points. In conclusion, these results suggest that coproantigen detection and PCR may be more sensitive alternatives to traditional methods such as EPG for diagnosis of Strongyloides venezuelensis infection.

Keywords

diagnosisfecal antigenimmunosuppressionPCRstrongyloidiasisStrongyloides venezuelensis
Type
Research Article
Information
Parasitology , Volume 142 , Issue 14 , December 2015 , pp. 1715 - 1721
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Copyright
Copyright © Cambridge University Press 2015 

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