Hostname: page-component-cd9895bd7-q99xh Total loading time: 0 Render date: 2024-12-27T02:54:10.307Z Has data issue: false hasContentIssue false
  • English
  • Français

Characterization of Trichomonas vaginalis haemolysis

Published online by Cambridge University Press:  06 April 2009

D. C. Dailey ,
C. Te-Hung  and
J. F. Alderete
D. C. Dailey
Affiliation:
Department of Microbiology, The University of Texas Health Science Center, San Antonio, Texas, USA.
C. Te-Hung
Affiliation:
Department of Microbiology, The University of Texas Health Science Center, San Antonio, Texas, USA.
J. F. Alderete
Affiliation:
Department of Microbiology, The University of Texas Health Science Center, San Antonio, Texas, USA.
Get access Rights & Permissions [Opens in a new window]

Extract

The haemolytic activity of live Trichomonas vaginalis organisms was investigated. Optimal haemolysis of human erythrocytes was observed at a parasite to erythrocyte ratio of 1:5 during a 2 h incubation period. No haemolytic activity was detected in concentrated culture supernatants after overnight growth of trichomonads or when parasites were separated from erythrocytes by a 3 μm filter, suggesting a contact-dependent mechanism for haemolysis. The haemolytic activity was temperature-dependent and maximal haemolysis occurred at 37 °C. Treatment of trichomonads with metronidazole reduced levels of haemolysis by > 50%. Maximal haemolysis occurred at the pH range of the vagina during trichomoniasis. N-μ-tosyl-L-lysyl-chloromethyl ketone and iodoacetamide, inhibitors of trichomonad cysteine proteinases, reduced the haemolytic activity of live parasites.

Keywords

Trichomonas vaginalishaemolytic activitymetronidazolecysteine proteinases
Type
Research Article
Information
Parasitology , Volume 101 , Issue 2 , October 1990 , pp. 171 - 175
Copyright
Copyright © Cambridge University Press 1990

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Alderete, J. F., Demeš, P., Gombošova, A., Valent, M., Fabušova, M., Janoška, A., Štefanović, J. & Arroyo, R. (1988). Specific parasitism of purified vaginal epithelial cells by Trichomonas vaginalis. Infection and Immunity 56, 2558–62.CrossRefGoogle ScholarPubMed
Alderete, J. F., Demeš, P., Gombošova, A., Valent, M., Janoška, A., Fabušova, H., Kasmala, L., Garza, G. E. & Metcalfe, E. C. (1987). Phenotypes and protein/epitope phenotypic variation among fresh isolates of Trichomonas vaginalis. Infection and Immunity 55, 1037–41.CrossRefGoogle ScholarPubMed
Alderete, J. F. & Garza, G. E. (1985). Specific nature of Trichomonas vaginalis parasitism of host cell surfaces. Infection and Immunity 50, 701–8.CrossRefGoogle ScholarPubMed
Alderete, J. F. & Garza, G. E. (1988). Identification and properties of Trichomonas vaginalis proteins involved in cytadherence. Infection and Immunity 56, 28–33.CrossRefGoogle ScholarPubMed
Alderete, J. F., Kasmala, L., Metcalfe, E. & Garza, G. E. (1986 a). Phenotypic variation and diversity among Trichomonas vaginalis and correlation of phenotype with trichomonal virulence determinants. Infection and Immunity 53, 285–93.CrossRefGoogle ScholarPubMed
Alderete, J. F. & Pearlman, E. (1984). Pathogenic Trichomonas vaginalis cytotoxicity to cell culture monolayers. British Journal of Venereal Disease 60, 99–105.Google ScholarPubMed
Alderete, J. F., Suprun-Brown, L. & Kasmala, L. (1986 b). Monoclonal antibody to a major surface glycoprotein immunogen differentiates isolates and subpopulations of Trichomonas vaginalis. Infection and Immunity 52, 70–5.CrossRefGoogle Scholar
Arroyo, R. & Alderete, J. F. (1989). Trichomonas vaginalis surface proteinase activity is necessary for parasite adherence to epithelial cells. Infection and Immunity 57, 2991–7.CrossRefGoogle ScholarPubMed
Brown, M. T. (1972). Trichomoniasis. Practitioner 209, 639–44.Google ScholarPubMed
Cohen, M. S., Black, J. R., Proter, R. A. & Sparling, D. F. (1984). Host defenses and vaginal mucosa. In Bacterial Vaginosis, (ed. Mardh, P. & Taylor Robinson, D.) pp. 13–22. Stockholm: Almquist and Wiksell.Google Scholar
Coombs, G. H. & North, M. J. (1983). An analysis of the proteinases of Trichomonas vaginalis by acrylamide gel electrophoresis. Parasitology 86, 1–6.CrossRefGoogle Scholar
Diamond, L. S. (1957). The establishment of various trichomonads of animals and man in axenic cultures. Journal of Parasitology 43, 488–90.CrossRefGoogle ScholarPubMed
Honigberg, B. M. (1978). Trichomonads of importance in human medicine. In Parasitic Protozoa, vol. 2 (ed. Kreier, J. P.) pp. 275–454. New York: Academic Press.Google Scholar
Jirovec, O. & Pertru, M. (1968). Trichomonas vaginalis and Trichomoniasis. Advances in Parasitology 6, 117–88.CrossRefGoogle ScholarPubMed
Krieger, J. N., Poisson, M. A. & Rein, M. F. (1983). Beta-hemolytic activity of Trichomonas vaginalis correlates with virulence. Infection and Immunity 41, 1291–5.CrossRefGoogle ScholarPubMed
Krieger, J. N., Ravdin, J. I. & Rein, M. F. (1985). Contact-dependent cytopathogenic mechanisms of Trichomonas vaginalis. Infection and Immunity 50, 778–86.CrossRefGoogle ScholarPubMed
Nelson, G. L. (1967). Lipid composition of erythrocytes in various mammalian species. Biochemica et Biophysica Acta 44, 221–32.CrossRefGoogle Scholar
Peterson, K. M. & Alderete, J. F. (1982). Host plasma proteins on the surface of pathogenic Trichomonas vaginalis. Infection and Immunity 37, 755–62.CrossRefGoogle ScholarPubMed
Peterson, K. M. & Alderete, J. F. (1984 a). Trichomonas vaginalis is dependent on uptake and degradation of human low density lipoproteins. Journal of Experimental Medicine 160, 1261–72.CrossRefGoogle ScholarPubMed
Peterson, K. M. & Alderete, J. F. (1984 b). Iron uptake and increased intracellular enzyme activity follow lactoferrin binding by Trichomonas vaginalis receptors. Journal of Experimental Medicine 160, 398–410.CrossRefGoogle ScholarPubMed
Pindak, F., Gardner, J. W. A. & Pindak, M. M. (1986). Growth and cytopathogenicity of Trichomonas vaginalis in tissue cultures. Journal of Clinical Microbiology 23, 672–8.CrossRefGoogle ScholarPubMed
Rennie, R. P., Freer, J. H. & Arbuthnott, J. P. (1974). The kinetics of erythrocyte lysis by Escherichia coli haemolysin. Journal of Medical Microbiology 7, 189–95.CrossRefGoogle ScholarPubMed