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Characterization of Megatrypanum trypanosomes from European Cervidae

Published online by Cambridge University Press:  06 April 2009

R. Böse ,
K. Petersen ,
H. Pospichal ,
N. Buchanan  and
A. Tait
R. Böse
Affiliation:
Institute of Parasitology, School of Veterinary Medicine, Bünteweg 17, 3000 Hannover 71, Germany
K. Petersen
Affiliation:
Institute of Parasitology, School of Veterinary Medicine, Bünteweg 17, 3000 Hannover 71, Germany
H. Pospichal
Affiliation:
Swiss Tropical Institute, Socinstrasse 57, 4002 Basel, Switzerland
N. Buchanan
Affiliation:
Wellcome Unit of Molecular Parasitology, Department of Veterinary Parasitology, University of Glasgow, Bearsden Road, Glasgow G61 IQH, UK
A. Tait
Affiliation:
Wellcome Unit of Molecular Parasitology, Department of Veterinary Parasitology, University of Glasgow, Bearsden Road, Glasgow G61 IQH, UK
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Summary

Megatrypanum trypanosomes have been isolated from a number of different European Cervidae, but on the basis of morphology it has not been possible to define the species to which these isolates belong. We isolated Trypanosoma (Megatrypanum) theileri from 10 cattle, and Megatrypanum trypanosomes from 11 fallow deer (Cervus dama), 9 red deer (Cervus elaphus), and 4 roe deer (Capreolus capreolus) by blood culture on a biphasic medium (NNN agar slopes). Trypanosomes were propagated in Schneider's Drosophila medium and characterized by isoenzyme analysis and molecular karyotyping. Isocitrate dehydrogenase and phosphoglucomutase were visualized after starch gel electrophoresis of trypanosome lysates. By cluster analysis of this data all isolates from deer were clearly separated from the T. (M.) theileri isolates from cattle. Isolates from roe deer were different not only from T. (M.) theileri but also from the other deer isolates. Isolates from fallow deer and red deer were grouped together. Thus, there are probably at least two different species of Megatrypanum trypanosomes in the three Cervidae. One parasitizing roe deer, the other, apparently less host specific species, infecting red deer and fallow deer. Separation of the chromosomes of Megatrypanum trypanosomes by pulsed-field gradient gel electrophoresis (PFGE) showed that each isolate contained a large number (> 18) of chromosomes ranging in size from 300 to > 2200 kb. The molecular karyotypes were similar for all isolates, although no isolate was identical to another.

Keywords

Megatrypanum trypanosomesTrypanosoma theileriisoenzyme analysismolecular karyotypeCervidae.
Type
Research Article
Information
Parasitology , Volume 107 , Issue 1 , July 1993 , pp. 55 - 61
Copyright
Copyright © Cambridge University Press 1993

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