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Characterization and localization of Schistosoma mansoni calreticulin Sm58

Published online by Cambridge University Press:  06 April 2009

J. Khalife ,
J. L. Liu ,
R. Pierce ,
E. Porchet ,
C. Godin  and
A. Capron
J. Khalife
Affiliation:
Centre d'Immunologie et de Biologie Parasitaire, INSERM U 167-CNRS 624, Institut Pasteur, 1 Rue de Professeur Calmette, B.P. 245, 59019 Lille Cédex, France
J. L. Liu
Affiliation:
Centre d'Immunologie et de Biologie Parasitaire, INSERM U 167-CNRS 624, Institut Pasteur, 1 Rue de Professeur Calmette, B.P. 245, 59019 Lille Cédex, France
R. Pierce
Affiliation:
Centre d'Immunologie et de Biologie Parasitaire, INSERM U 167-CNRS 624, Institut Pasteur, 1 Rue de Professeur Calmette, B.P. 245, 59019 Lille Cédex, France
E. Porchet
Affiliation:
Centre d'Immunologie et de Biologie Parasitaire, INSERM U 167-CNRS 624, Institut Pasteur, 1 Rue de Professeur Calmette, B.P. 245, 59019 Lille Cédex, France
C. Godin
Affiliation:
Centre d'Immunologie et de Biologie Parasitaire, INSERM U 167-CNRS 624, Institut Pasteur, 1 Rue de Professeur Calmette, B.P. 245, 59019 Lille Cédex, France
A. Capron
Affiliation:
Centre d'Immunologie et de Biologie Parasitaire, INSERM U 167-CNRS 624, Institut Pasteur, 1 Rue de Professeur Calmette, B.P. 245, 59019 Lille Cédex, France
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Summary

Recombinant Schistosoma mansoni calreticulin (SmCaR) was expressed in Escherichia coli, using the glutathione S-transferase fusion protein, and its Ca2+-binding capacity was determined. Results obtained by a 45Ca2+ overlay technique showed that Ca2+-binding site(s) were present in the recombinant CaR indicating that proper folding of the protein was obtained using this system. An antiserum raised against the recombinant SmCaR showed that the native protein (Sm58) was expressed in all stages of the life-cycle from cercariae to the adult worm and in the egg. However, SmCaR seems to be a developmentally regulated protein whose expression can be used to study the post-transformational differentiation of the schistosomulum. Localization of SmCaR demonstrated that the majority of SmCaR was expressed in the epithelia of the digestive duct and in the genital organs. These results suggest that SmCaR, by regulating the Ca2+ concentration, may play an important role during cell proliferation. Finally the presence of SmCaR in miracidia and in the genital organs suggests that the antibody response directed against this protein could interfere in egg production.

Keywords

Schistosoma mansonicalreticulinCa2+-binding protein
Type
Research Article
Information
Parasitology , Volume 108 , Issue 5 , June 1994 , pp. 527 - 532
Copyright
Copyright © Cambridge University Press 1994

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