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Ceratomyxa gracillima n. sp. (Cnidaria: Myxosporea) provides evidence of panmixia and ceratomyxid radiation in the Amazon basin

Published online by Cambridge University Press:  17 January 2018

Suellen A. Zatti ,
Stephen D. Atkinson ,
Antônio A. M. Maia ,
Jerri L. Bartholomew  and
Edson A. Adriano
Suellen A. Zatti
Affiliation:
Department of Animal Biology, Institute of Biology, University of Campinas, Caixa Postal 6109, CEP 13083-970, Campinas, SP, Brazil
Stephen D. Atkinson
Affiliation:
Department of Microbiology, Oregon State University, Corvallis, Oregon, USA
Antônio A. M. Maia
Affiliation:
Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, São Paulo University, Avenida Duque de Caxias Norte, 225, CEP 13635-900, Pirassununga, SP, Brazil
Jerri L. Bartholomew
Affiliation:
Department of Microbiology, Oregon State University, Corvallis, Oregon, USA
Edson A. Adriano*
Affiliation:
Department of Animal Biology, Institute of Biology, University of Campinas, Caixa Postal 6109, CEP 13083-970, Campinas, SP, Brazil Department of Ecology and Evolutionary Biology, Federal University of São Paulo, Rua Professor Arthur Riedel, 275, Jardim Eldorado, CEP 09972-270, Diadema, SP, Brazil
*
Author for correspondence: Edson A. Adriano, E-mail: [email protected]
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Abstract

We describe a new freshwater myxosporean species Ceratomyxa gracillima n. sp. from the gall bladder of the Amazonian catfish Brachyplatystoma rousseauxii; the first myxozoan recorded in this host. The new Ceratomyxa was described on the basis of its host, myxospore morphometry, ssrDNA and internal transcribed spacer region (ITS-1) sequences. Infected fish were sampled from geographically distant localities: the Tapajós River, Pará State, the Amazon River, Amapá State and the Solimões River, Amazonas State. Immature and mature plasmodia were slender, tapered at both ends, and exhibited vermiform motility. The ribosomal sequences from parasite isolates from the three localities were identical, and distinct from all other Ceratomyxa sequences. No population-level genetic variation was observed, even in the typically more variable ITS-1 region. This absence of genetic variation in widely separated parasite samples suggests high gene flow as a result of panmixia in the parasite populations. Maximum likelihood and maximum parsimony analyses placed C. gracillima n. sp. sister to Ceratomyxa vermiformis in a subclade together with Ceratomyxa brasiliensis and Ceratomyxa amazonensis, all of which have Amazonian hosts. This subclade, together with other Ceratomyxa from freshwater hosts, formed an apparently early diverging lineage. The Amazonian freshwater Ceratomyxa species may represent a radiation that originated during marine incursions into the Amazon basin that introduced an ancestral lineage in the late Oligocene or early Miocene.

Keywords

Amazon basingene flowhost migrationITS-1 sequencingmarine incursionsssrDNA
Type
Research Article
Information
Parasitology , Volume 145 , Issue 9 , August 2018 , pp. 1137 - 1146
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Copyright
Copyright © Cambridge University Press 2018 

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