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A battery of 12 microsatellite markers for genetic analysis of the Leishmania (Viannia) guyanensis complex

Published online by Cambridge University Press:  07 July 2010

V. ROUGERON ,
T. DE MEEÛS ,
M. HIDE ,
E. WALECKX ,
J. DEREURE ,
J. AREVALO ,
A. LLANOS-CUENTAS  and
A. L. BAÑULS
V. ROUGERON*
Affiliation:
Génétique et Evolution des Maladies Infectieuses, IRD/CNRS/UMI (UMR 2724), Montpellier, F-34394, France
T. DE MEEÛS
Affiliation:
UMR 177 IRD-CIRAD “Laboratoire de Recherches et de Coordination sur les Trypanosomoses”, Centre International de Recherche-Développement sur l'Elevage en zone Subhumide (Cirdes), 01 BP 454, Bobo-Dioulasso 01, Burkina-Faso CNRS, Délégation Languedoc-Roussillon, 1919, route de Mende – 34293 Montpellier cedex 5, France
M. HIDE
Affiliation:
Génétique et Evolution des Maladies Infectieuses, IRD/CNRS/UMI (UMR 2724), Montpellier, F-34394, France
E. WALECKX
Affiliation:
UR016 IRD, INLASA, Laboratoire d'entomologie médicale de La Paz, Bolivie
J. DEREURE
Affiliation:
Génétique et Evolution des Maladies Infectieuses, IRD/CNRS/UMI (UMR 2724), Montpellier, F-34394, France Laboratoire de Parasitologie – Mycologie, Montpellier, F 34090, France
J. AREVALO
Affiliation:
Instituto de medicina Tropical “Alexander von Humboldt,” Lima, Peru
A. LLANOS-CUENTAS
Affiliation:
Instituto de medicina Tropical “Alexander von Humboldt,” Lima, Peru
A. L. BAÑULS
Affiliation:
Génétique et Evolution des Maladies Infectieuses, IRD/CNRS/UMI (UMR 2724), Montpellier, F-34394, France
*
*Corresponding author: Laboratoire GEMI, UMR IRD/CNRS/UMI 2724, Centre IRD, 911, avenue Agropolis BP 64501, 34394 Montpellier Cedex 5, France. Tel: +33 4 67 41 62 26. Fax: +33 4 67 41 62 99. E-mail: rougeron.virginie@ gmail.com
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Summary

We used 12 microsatellite markers developed for Leishmania braziliensis to genotype 28 strains of the main species of the Leishmania guyanensis complex (i.e. L. guyanensis and L. panamensis) collected in Ecuador and Peru. The important heterozygote deficits observed in these populations are similar with the previous data obtained in L. braziliensis and raise again the debate on the reproductive mode of these protozoan parasites. The data showed genetic polymorphism and geographical differentiation giving information on population structure of the L. guyanensis complex. Regarding the two species, this study enhances again the debate on the taxonomic status of the different isolates belonging to L. guyanensis s.l. since the results showed substantial heterogeneity within this species complex. In conclusion, this study increases the number of available microsatellite loci for L. guyanensis species complex and raises fundamental biological questions. It confirms that microsatellite markers constitute good tools for population genetic studies on parasites of this complex.

Keywords

Leishmania guyanensis complexmicrosatellite markerspopulation geneticspopulation structure
Type
Research Article
Information
Parasitology , Volume 137 , Issue 13 , November 2010 , pp. 1879 - 1884
Copyright
Copyright © Cambridge University Press 2010

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