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Assessment of parasite population dynamics in mixed infections of rodent plasmodia

Published online by Cambridge University Press:  06 April 2009

G. Snounou ,
T. Bourne ,
W. Jarra ,
S. Viriyakosol ,
J. C. Wood  and
K. N. Brown
G. Snounou
Affiliation:
Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
T. Bourne
Affiliation:
Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
W. Jarra
Affiliation:
Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
S. Viriyakosol
Affiliation:
Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
J. C. Wood
Affiliation:
Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
K. N. Brown
Affiliation:
Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
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Summary

Cloned lines of the four rodent Plasmodium species can be differentiated by the RFLP pattern generated following Southern blotting and probing with PCsv4.1, a probe derived from a P. chabaudi chabaudi genomic library. Groups of CBA/Ca mice were inoculated simultaneously with cloned lines from two parasite species or strains. Six mixed species and three mixed strain infections using rodent malaria lines were initiated. The composition of the parasite population in each group was determined qualitatively and semi-quantitatively by analysis of the DNA purified from daily blood samples, thereby providing a dynamic representation of each mixed infection. Effects on the course of parasitaemias are presented and discussed.

Keywords

DNA probemixed infectionrodent malariaPlasmodium chabaudiPlasmodium bergheiPlasmodium vinckeiPlasmodium yoelii
Type
Research Article
Information
Parasitology , Volume 105 , Issue 3 , December 1992 , pp. 363 - 374
Copyright
Copyright © Cambridge University Press 1992

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