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Antioxidants promote establishment of trypanosome infections in tsetse

Published online by Cambridge University Press:  19 February 2007

E. T. MacLEOD ,
I. MAUDLIN ,
A. C. DARBY  and
S. C. WELBURN
E. T. MacLEOD
Affiliation:
Centre for Infectious Diseases, College of Medicine and Veterinary Medicine, The University of Edinburgh, Easter Bush Veterinary Centre, Roslin, Midlothian EH25 9RG, UK
I. MAUDLIN
Affiliation:
Centre for Infectious Diseases, College of Medicine and Veterinary Medicine, The University of Edinburgh, Easter Bush Veterinary Centre, Roslin, Midlothian EH25 9RG, UK
A. C. DARBY
Affiliation:
Centre for Infectious Diseases, College of Medicine and Veterinary Medicine, The University of Edinburgh, Easter Bush Veterinary Centre, Roslin, Midlothian EH25 9RG, UK
S. C. WELBURN*
Affiliation:
Centre for Infectious Diseases, College of Medicine and Veterinary Medicine, The University of Edinburgh, Easter Bush Veterinary Centre, Roslin, Midlothian EH25 9RG, UK
*
*Corresponding author. Tel: +44 131 650 6228. Fax: +44 131 650 7348. E-mail: [email protected]
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Summary

Efficient, cyclical transmission of trypanosomes through tsetse flies is central to maintenance of human sleeping sickness and nagana across sub-Saharan Africa. Infection rates in tsetse are normally very low as most parasites ingested with the fly bloodmeal die in the fly gut, displaying the characteristics of apoptotic cells. Here we show that a range of antioxidants (glutathione, cysteine, N-acetyl-cysteine, ascorbic acid and uric acid), when added to the insect bloodmeal, can dramatically inhibit cell death of Trypanosoma brucei brucei in tsetse. Both L- and D-cysteine invoked similar effects suggesting that inhibition of trypanosome death is not dependent on protein synthesis. The present work suggests that antioxidants reduce the midgut environment protecting trypanosomes from cell death induced by reactive oxygen species.

Keywords

Trypanosoma brucei bruceitrypanosomiasistsetseantioxidantapoptosis
Type
Research Article
Information
Parasitology , Volume 134 , Issue 6 , June 2007 , pp. 827 - 831
Copyright
Copyright © Cambridge University Press 2007

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