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Antioxidant defense of Nrf2 vs pro-inflammatory system of NF-κB during the amoebic liver infection in hamster

Published online by Cambridge University Press:  23 November 2016

LISETH R. ALDABA-MURUATO ,
MARTIN H. MUÑOZ-ORTEGA ,
MARÍA DEL R. CAMPOS-ESPARZA ,
JOSÉ R. MACÍAS-PÉREZ ,
NAYELI A. MÁRQUEZ-MUÑOZ ,
ANA G. VILLALOBOS-SANTOS  and
JAVIER VENTURA-JUÁREZ
LISETH R. ALDABA-MURUATO
Affiliation:
Departamento de Morfología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes, Ags., México
MARTIN H. MUÑOZ-ORTEGA
Affiliation:
Departamento de Química, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes, Ags., México
MARÍA DEL R. CAMPOS-ESPARZA
Affiliation:
Departamento de Morfología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes, Ags., México
JOSÉ R. MACÍAS-PÉREZ
Affiliation:
Departamento de Morfología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes, Ags., México
NAYELI A. MÁRQUEZ-MUÑOZ
Affiliation:
Departamento de Morfología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes, Ags., México
ANA G. VILLALOBOS-SANTOS
Affiliation:
Departamento de Morfología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes, Ags., México
JAVIER VENTURA-JUÁREZ*
Affiliation:
Departamento de Morfología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes, Ags., México
*
*Corresponding author: Departamento de Morfología, Universidad Autónoma de Aguascalientes, Centro de Ciencias Básicas, Ed. 202. Av. Universidad # 940, Ciudad Universitaria, C. P. 20131, Aguascalientes, Ags., México. E-mail: [email protected]
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Summary

Entamoeba histolytica is the causative agent of amoebic liver abscess (ALA), which course with an uncontrolled inflammation and nitro-oxidative stresses, although it is well known that amoeba has an effective defence mechanisms against this toxic environment, the underlying molecular factors responsible for progression of tissue damage remain largely unknown. The purpose of the present study was to determine during the acute stage of ALA in hamsters, the involvement of nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and nuclear factor-kappa B (NF-κB), which are activated in response to oxidative stress. From 12 h post-infection the ALA was visible, haematoxylin-eosin and Masson's trichrome stains were consistent with these observations, and alanine aminotransferase, alkaline phosphatase and γ-glutamyl transpeptidase serum activities were increased too. At 48 h after infection, liver glycogen content was significantly reduced. Western blot analyses showed that 4-Hydroxy-2-nonenal peaked at 12 h, while glycogen synthase kinase-3β, cleaved caspase-3, pNF-κB, interleukin-1β and tumour necrosis factor-α were overexpressed from 12 to 48 h post-infection. Otherwise, Nrf2 and superoxide dismutase-1, decreased at 48 h and catalase declined at 36 and 48 h. Furthermore, heme oxygenase-1 was increased at 12 and 24 h and decreased to normal levels at 36 and 48 h. These findings suggest for the first time that the host antioxidant system of Nrf2 is influenced during ALA.

Keywords

E. histolyticaamoebic liver abscessoxidative stressNrf2NF-kappa BGSK-3βcleaved caspase-3
Type
Research Article
Information
Parasitology , Volume 144 , Issue 4 , April 2017 , pp. 384 - 393
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Copyright
Copyright © Cambridge University Press 2016 

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