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Antigenic characterization of adult Wuchereria bancrofti filarial nematodes

Published online by Cambridge University Press:  06 April 2009

T. M. Morgan ,
Inge Sutanto ,
Purnomo ,
Sukartono ,
F. Partono  and
R. M. Maizels
T. M. Morgan
Affiliation:
Department of Pure and Applied Biology, Imperial College of Science and Technology, Prince Consort Road, London SW7 2BB, UK
Inge Sutanto
Affiliation:
Department of Parasitology, Faculty of Medicine, University of Jakarta, Salemba Raya 6, Jakarta, ID
Purnomo
Affiliation:
Department of Parasitology, Faculty of Medicine, University of Jakarta, Salemba Raya 6, Jakarta, ID
Sukartono
Affiliation:
Department of Parasitology, Faculty of Medicine, University of Jakarta, Salemba Raya 6, Jakarta, ID
F. Partono
Affiliation:
Department of Parasitology, Faculty of Medicine, University of Jakarta, Salemba Raya 6, Jakarta, ID
R. M. Maizels
Affiliation:
Department of Pure and Applied Biology, Imperial College of Science and Technology, Prince Consort Road, London SW7 2BB, UK
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Summary

Adult Wuchereria bancrofti were recovered from infected Presbytis cristatus monkeys and radio-isotope labelled extrinsically with 125I and in vitro with [35S]methionine. 125I labelling of the surface of adult W. bancrofti permitted a comparison between the major surface antigens of this species and those from the related lymphatic filariae, Brugia malayi and B. pahangi. All species bear a prominent Mr 29 000 surface antigen but among the differences observed were the strongly labelled molecules with Mr 58 000 and 67 000 in W. bancrofti which are extremely faint in the Brugia species. The [35S]methionine label was effectively incorporated into somatic parasite proteins in vitro although it was not possible to identify any secreted proteins in this way. The antigenicity of these products was investigated using a variety of sera from homologous and heterologous infections and the immunoprecipitation patterns highlighted particular differences between somatic proteins of male and female worms. One secreted antigen was detected, however, by virtue of its phosphorylcholine epitopes, in the culture medium of mixed adult worms; medium from male W. bancrofti adults was negative although homogenates of either sex of adult W. bancrofti were strongly positive in the same system.

Type
Research Article
Information
Parasitology , Volume 93 , Issue 3 , December 1986 , pp. 559 - 569
Copyright
Copyright © Cambridge University Press 1986

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