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Anthelmintic metabolism in parasitic helminths: proteomic insights

Published online by Cambridge University Press:  10 July 2012

PETER M. BROPHY ,
NEIL MACKINTOSH  and
RUSSELL M. MORPHEW
PETER M. BROPHY*
Affiliation:
Parasitology Group, Institute of Biological, Environmental and Rural Sciences (IBERS), Aberystwyth University, Aberystwyth, Ceredigion, Wales SY23 3FG, UK
NEIL MACKINTOSH
Affiliation:
Parasitology Group, Institute of Biological, Environmental and Rural Sciences (IBERS), Aberystwyth University, Aberystwyth, Ceredigion, Wales SY23 3FG, UK
RUSSELL M. MORPHEW
Affiliation:
Parasitology Group, Institute of Biological, Environmental and Rural Sciences (IBERS), Aberystwyth University, Aberystwyth, Ceredigion, Wales SY23 3FG, UK
*
*Corresponding author: IBERS, Aberystwyth University, Aberystwyth SY23 3FG, [email protected] Tel: +44(0)1970 622332
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Summary

Anthelmintics are the cornerstone of parasitic helminth control. Surprisingly, understanding of the biochemical pathways used by parasitic helminths to detoxify anthelmintics is fragmented, despite the increasing global threat of anthelmintic resistance within the ruminant and equine industries. Reductionist biochemistry has likely over-estimated the enzymatic role of glutathione transferases in anthelmintic metabolism and neglected the potential role of the cytochrome P-450 superfamily (CYPs). Proteomic technologies offers the opportunity to support genomics, reverse genetics and pharmacokinetics, and provide an integrated insight into both the cellular mechanisms underpinning response to anthelmintics and also the identification of biomarker panels for monitoring the development of anthelmintic resistance. To date, there have been limited attempts to include proteomics in anthelmintic metabolism studies. Optimisations of membrane, post-translational modification and interaction proteomic technologies in helminths are needed to especially study Phase I CYPs and Phase III ABC transporter pumps for anthelmintics and their metabolites.

Keywords

Proteomicshelminthanthelminticanthelmintic resistancexenobiotic
Type
Research Article
Information
Parasitology , Volume 139 , Issue 9: Symposia of the British Society for Parasitology Volume 48 Proteomic insights into parasite biology , August 2012 , pp. 1205 - 1217
Copyright
Copyright © Cambridge University Press 2012

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