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Analysis of the complete sequence of a muscle calcium-binding protein of Schistosoma mansoni*

Published online by Cambridge University Press:  06 April 2009

T. J. Stewart ,
A. L. Smith  and
J. C. Havercroft
T. J. Stewart
Affiliation:
Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP
A. L. Smith
Affiliation:
Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP
J. C. Havercroft
Affiliation:
Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP
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Summary

The complete sequence of the cDNA encoding a 20 kDa calcium-binding protein of Schistosoma mansoni (Sm20) has been determined. The predicted amino acid sequence contains 4 EF hand domains but examination of the predicted secondary structure of Sm20, together with the specific residues in each calcium-binding domain, suggests that only 1 EF hand (domain IV) is functional. Sm20 is most homologous to calmodulin, troponin C and the regulatory light-chain of myosin, particularly those of invertebrates. However, troponin C and the regulatory light-chain of myosin can be distinguished from Sm20 by size and by their differential levels of expression during the life-cycle. Sm20 also appears to be distinct from calmodulin but may be functionally equivalent to the soluble sarcoplasmic calcium-binding proteins of molluscs and crustacea which may act as a reservoir for calcium in muscle. Sm20 is encoded by a small multi-gene family whose members are clustered within a 15 kb region of the genome. A 20 kDa antigen, cross-reactive with Sm20, is expressed in Schistosoma haematobium, Fasciola hepatica and Paragonomus mexicanus.

Keywords

Schistosoma mansonicalciummusclesequence.
Type
Research Article
Information
Parasitology , Volume 105 , Issue 3 , December 1992 , pp. 399 - 408
Copyright
Copyright © Cambridge University Press 1992

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References

REFERENCES

Andresen, K., Tom, T. D. & Strand, M. (1991). Characterisation of cDNA clones encoding a novel calcium-activated neutral proteinase from Schistosoma mansoni. Journal of Biological Chemistry 266, 15085–90.CrossRefGoogle ScholarPubMed
Cordingley, J. S., Taylor, D. W., Dunne, D. W. & Butterworth, A. E. (1983). Clone banks of cDNA from the parasite Schistosoma mansoni: isolation of clones containing a potentially immunodiagnostic antigen. Gene 26, 25–39.CrossRefGoogle ScholarPubMed
Da Silva, A. C. R. & Reinach, F. C. (1991). Calcium binding induces conformational changes in muscle regulatory proteins. Trends in Biochemical Sciences 16, 53–7.CrossRefGoogle ScholarPubMed
Demaille, J. G. (1982). Calmodulin and calcium-binding proteins: evolutionary diversification of structure and function. Calcium and Cell Function 2, 111–39.CrossRefGoogle Scholar
Fuhrman, J. A. (1990). Calcium binding proteins in schistosomes. Parasitology Today 6, 172–3.CrossRefGoogle ScholarPubMed
Gerday, CH. (1988). Soluble calcium binding proteins in vertebrate and invertebrate muscle. In Calcium and Calcium Binding Proteins (ed. Gerday, Ch., Bolis, L. & Gilles, R.), pp. 23–39. Heidelberg: Springer-Verlag.CrossRefGoogle Scholar
Grossman, Z., Ram, D., Markovics, A., Tarab-Hazdai, R., Lantner, F., Ziv, E. & Schechter, I. (1990). Schistosoma mansoni: stage-specific expression of muscle-specific genes. Experimental Parasitology 70, 62–71.CrossRefGoogle ScholarPubMed
Havercroft, J. C. & Cleveland, D. W. (1984). Programmed expression of β-tubulin genes during development and differentiation of the chicken. Journal of Cell Biology 99, 1927–35.CrossRefGoogle ScholarPubMed
Havercroft, J. C., Huggins, M. C., Nene, V., Dunne, D. W., Richardson, B. A., Taylor, D. W. & Butterworth, A. E. (1988). Cloning of the gene encoding a 50 kilodalton potential surface antigen of Schistosoma mansoni. Molecular and Biochemical Parasitology 30, 83–8.CrossRefGoogle ScholarPubMed
Havercroft, J. C., Huggins, M. C., Dunne, D. W. & Taylor, D. W. (1990). Characterisation of Sm20, a 20-kilodalton calcium-binding protein of Schistosoma mansoni. Molecular and Biochemical Parasitology 38, 211–20.CrossRefGoogle ScholarPubMed
Havercroft, J. C., Smith, A. L. & Williams, R. H. (1991). Schistosoma mansoni: immuno-localization of the calcium binding protein Sm20. Parasite Immunology 13, 593–604.CrossRefGoogle ScholarPubMed
Heizmann, C. W. & Hunziker, W. (1990). Intracellular calcium-binding molecules. In Intracellular Calcium Regulation (ed. Bronner, F.), pp. 218–48. New York: Alan R. Liss.Google Scholar
Heizmann, C. W. & Hunziker, W. (1991). Intracellular calcium-binding proteins: more sites than insights. Trends in Biochemical Sciences 16, 98–103.CrossRefGoogle ScholarPubMed
Kozak, M. (1986). Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell 44, 283–92.CrossRefGoogle ScholarPubMed
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227, 680–4.CrossRefGoogle ScholarPubMed
Meadows, H. M. & Simpson, A. J. G. (1989). Codon usage in Schistosoma. Molecular and Biochemical Parasitology 36, 291–9.CrossRefGoogle ScholarPubMed
Moncrief, N. D., Kretsinger, R. H. & Goodman, M. (1990). Evolution of EF-hand calcium-modulated proteins. I. Relationships based on amino acid sequences. Journal of Molecular Evolution 30, 522–62.CrossRefGoogle ScholarPubMed
Moser, D., Doenhoff, M. J. & Klinkert, M.-Q. (1992). A stage-specific calcium-binding protein expressed in eggs of Schistosoma mansoni. Molecular and Biochemical Parasitology 51, 229–38.CrossRefGoogle ScholarPubMed
Ram, D., Grossman, Z., Markovics, A., Avivi, A., Ziv, E., Lantner, F. & Schechter, I. (1989). Rapid changes in the expression of a gene encoding a calcium-binding protein in Schistosoma mansoni. Molecular and Biochemical Parasitology 34, 167–76.CrossRefGoogle ScholarPubMed
Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, 2nd edn.Cold Spring Harbour, New York: Cold Spring Harbor Laboratory Press.Google Scholar
Sanger, F., Nicklen, S. & Coulson, A. R. (1977). DNA sequencing with chain terminating inhibitors. Proceedings of the National Academy of Sciences, USA 74, 5463–7.CrossRefGoogle ScholarPubMed
Shank, P. R., Hughes, S. H., Kung, H. J., Majors, H. J., Quintrell, N., Guntaka, R. V., Bishop, J. M. & Varmus, H. E. (1978). Mapping of unintegrated avian sarcoma virus: termini of linear DNA of 300 nucleotides present once or twice in two species of linear DNA. Cell 15, 1385–95.CrossRefGoogle ScholarPubMed
Siddiqui, A. A., Podesta, R. B. & Clarke, M. W. (1991). Schistosoma mansoni: characterization and identification of calcium-binding proteins associated with the apical plasma membrane and envelope. Experimental Parasitology 72, 63–8.CrossRefGoogle ScholarPubMed
Smith, A. L., Huggins, M. C. & Havercroft, J. C. (1992). Schistosoma mansoni: adult males and females differentially express antigens encoded by repetitive genomic DNA. Parasitology 105, 63–9.CrossRefGoogle ScholarPubMed
Smithers, S. R. & Terry, R. J. (1965). The infection of laboratory hosts with cercariae of Schistosoma mansoni and the recovery of adult worms. Parasitology 55, 695–700.CrossRefGoogle ScholarPubMed
Southern, E. M. (1975). Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Evolution 98, 503–17.CrossRefGoogle ScholarPubMed
Taylor, D. W. & Wells, P. Z. (1984). Isolation and antigen analysis of surface tegument membranes from schistosomula of Schistosoma mansoni. Parasitology 89, 495–510.CrossRefGoogle ScholarPubMed
Thompson, D. P., Chen, G.-Z., Sample, A. K., Semeyn, D. R. & Bennett, J. L. (1980). Calmodulin: biochemical, physiological and morphological effects on Schistosoma mansoni. American Journal of Physiology 251, 1051–8.Google Scholar