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Analysis of mixed parasite populations of Theileria sergenti using cDNA probes encoding a major piroplasm surface protein

Published online by Cambridge University Press:  06 April 2009

T. Matsuba ,
H. Kubota ,
M. Tanaka ,
M. Hattori ,
M. Murata ,
C. Sugimoto  and
M. Onuma
T. Matsuba
Affiliation:
Department of Epizootiology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060
H. Kubota
Affiliation:
Department of Epizootiology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060
M. Tanaka
Affiliation:
Department of Epizootiology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060 Kyoto Biken Laboratories, Uji, Kyoto 611, Japan
M. Hattori
Affiliation:
Department of Epizootiology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060
M. Murata
Affiliation:
Department of Epizootiology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060
C. Sugimoto
Affiliation:
Department of Epizootiology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060
M. Onuma
Affiliation:
Department of Epizootiology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060
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Summary

The gene for the 32 kDa surface protein (p32) of Theileria sergenti was cloned into λgt11 and its nucleotide sequence was determined. The gene encodes a protein of 283 amino acids as deduced from its nucleotide sequence with a 22 residue N-terminal signal peptide. Using this cDNA as a probe we have isolated another two clones from a cDNA library with a CDM8 vector system derived from the same parasite stock. Comparison with three cDNA clones revealed differential polyadenylation and differences in sequences of non-coding regions. Within the coding regions, there were nucleotide transitions which affected the Pst I-restriction site, and one of the transitions was also accompanied by an amino acid substitution (Ala to Gly). Southern blot analysis showed hybridization pattern changes among the parasites isolated from individual calves at different times after infection. From these results, we conclude that at least 3 genetically different parasite populations may coexist, and that transition to predominant parasite populations might occur during persistent infections in a host, possibly to evade the host immune responses.

Keywords

Theileria sergentimixed populationamino acid substitutioncattle
Type
Research Article
Information
Parasitology , Volume 107 , Issue 4 , November 1993 , pp. 369 - 377
Copyright
Copyright © Cambridge University Press 1993

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