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Analysis of Leishmania mimetic neoglycoproteins for the cutaneous leishmaniasis diagnosis

Published online by Cambridge University Press:  28 May 2018

Lígia Moraes Barizon de Souza ,
Vanete Thomaz Soccol ,
Ricardo Rasmussen Petterle ,
Michelle D. Bates  and
Paul A. Bates
Lígia Moraes Barizon de Souza
Affiliation:
Laboratory of Molecular Biology, Department of Bioprocess Engineering and Biotechnology, Universidade Federal do Paraná, Avenida Coronel Francisco Heráclito dos Santos, 210 – Usina Piloto B, Curitiba, Paraná 81531-970, Brazil
Vanete Thomaz Soccol
Affiliation:
Laboratory of Molecular Biology, Department of Bioprocess Engineering and Biotechnology, Universidade Federal do Paraná, Avenida Coronel Francisco Heráclito dos Santos, 210 – Usina Piloto B, Curitiba, Paraná 81531-970, Brazil
Ricardo Rasmussen Petterle
Affiliation:
Nucleus of Medical Education, Department of Community Health, Universidade Federal do Paraná, Rua Padre Camargo, 280 – 7th floor, Curitiba, Paraná 80060-240, Brazil
Michelle D. Bates
Affiliation:
Division of Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, Lancaster, Lancashire, LA1 4YQ, UK
Paul A. Bates*
Affiliation:
Division of Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, Lancaster, Lancashire, LA1 4YQ, UK
*
Author for correspondence: Paul A. Bates, E-mail: [email protected]
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Abstract

Oligosaccharides are broadly present on Leishmania cell surfaces. They can be useful for the leishmaniases diagnosis and also helpful in identifying new cell markers for the disease. The disaccharide Galα1-3Galβ is the immunodominant saccharide in Leishmania cell surface and is the unique non-reducing terminal glycosphingolipids structure recognized by anti-α-Gal. This study describes an enzyme-linked immunosorbent assay (ELISA) used to measure serum levels of anti-α-galactosyl (α-Gal) antibodies in patients with cutaneous leishmaniasis (CL). Optimal ELISA conditions were established and two neoglycoproteins (NGP) containing the Galα1-3Gal terminal fraction (Galα1-3Galβ1-4GlcNAc-HAS and Galα1-3Gal-HAS) and one Galα1-3Gal NGP analogue (Galα1-3Galβ1-3GlcNAc-HAS) were used as antigens. Means of anti-α-Gal antibody titres of CL patients were significantly higher (P < 0.05) than the healthy individuals for all NGPs tested. Sensitivity and specificity of all NGPs ranged from 62.2 to 78.4% and 58.3 to 96.7%, respectively. In conclusion, the NGPs can be used for CL diagnosis.

Keywords

α-galactosylantigenELISAL. braziliensissaccharide
Type
Research Article
Information
Parasitology , Volume 145 , Issue 14 , December 2018 , pp. 1938 - 1948
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Copyright
Copyright © Cambridge University Press 2018 

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