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An integrative approach assesses the intraspecific variations of Procamallanus (Spirocamallanus) inopinatus, a common parasite in Neotropical freshwater fishes, and the phylogenetic patterns of Camallanidae

Published online by Cambridge University Press:  14 September 2020

Lorena G. Ailán-Choke ,
Luiz E.R. Tavares ,
José L. Luque  and
Felipe B. Pereira Open the ORCID record for Felipe B. Pereira [Opens in a new window]
Lorena G. Ailán-Choke
Affiliation:
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto para el Estudio de la Biodiversidad de Invertebrados, Facultad de Ciencias Naturales, Universidad Nacional de Salta, Av. Bolivia 5150, (4400) Salta, Argentina
Luiz E.R. Tavares
Affiliation:
Departamento de Patologia, Instituto de Biociências, Universidade Federal de Mato Grosso do Sul, Av. Costa e Silva s/n°, CEP 79070-900, Campo Grande, MS, Brasil
José L. Luque
Affiliation:
Departamento de Parasitologia Animal, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, BR 465, Km 47, CEP 23851-970, Seropédica, RJ, Brasil
Felipe B. Pereira*
Affiliation:
Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Presidente Antônio Carlos, 6627, Pampulha, CEP 31270-901, Belo Horizonte, MG, Brasil
*
Author for correspondence: Prof. Dr Felipe Bisaggio Pereira, E-mail: [email protected]
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Abstract

Integrative taxonomy was used to evaluate two component populations of Procamallanus (Spirocamallanus) inopinatus in Brazil and the phylogeny Camallanidae. Parasite populations were collected in the characiform Anostomoides passionis from River Xingu (Amazon basin) and Megaleporinus elongatus from River Miranda (Paraguay basin). Morphology was analysed using light and scanning electron microscopy (SEM). Genetic characterization was based on partial sequences of the 18S and 28S rDNA, and COI mtDNA. Phylogenies were based on 18S and COI due to data availability. Generalized Mixed Yule Coalescent (GMYC), Poisson Tree Process (PTP) and *BEAST were used for species delimitation and validation. SEM revealed for the first time the presence of minute denticles and pore-like structures surrounding the oral opening, phasmids in females and confirmed other important morphological aspects. Statistical comparison between the two-component populations indicated morphometric variations, especially among males. The different component population of P. (S.) inopinatus showed variable morphometry, but uniform morphology and were validated as conspecific by the GMYC, PTP and *BEAST. Some camallanid sequences in GenBank have incorrect taxonomic labelling. Host, environment and geographic aspects seem to be related to some lineages within Camallanidae; however, their real phylogenetic meanings are still unclear.

Keywords

AnostomidaeCharaciformesendoparasitehelminthintegrative taxonomymorphologyNematodagenetic characterization
Type
Research Article
Information
Parasitology , Volume 147 , Issue 14 , December 2020 , pp. 1752 - 1764
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Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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