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An in vitro study of the effect of oxygen tension upon the motility of Nippostrongylus brasiliensis

Published online by Cambridge University Press:  06 April 2009

Thomas J. W. Alphey
Thomas J. W. Alphey
Affiliation:
Department of Zoology, University College, Cardiff*
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Extract

An activity cell apparatus to facilitate quantitative measurements of motility of small nematodes in saline is described. The effects of salines with a pO2 either greater than 120 mmHg or less than 12 mmHg upon the motility of adult N. brasiliensis females was studied. The worms were found to require an oxygen tension greater than 12 mmHg to be able to maintain their motility.

I am grateful to Professors J. Brough and D. Bellamy for providing the facilities during the course of this study. I should also like to thank Dr R. A.Hammond for his supervision, help and criticism throughout the project, and Mr C. Welsby for his technical assistance in the construction of the activity cell.

This work was carried out during the tenure of a Scientific Research Council Studentship.

Type
Research Article
Information
Parasitology , Volume 64 , Issue 2 , April 1972 , pp. 181 - 186
Copyright
Copyright © Cambridge University Press 1972

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References

REFERENCES

Alphey, T. J. W. (1970). Studies on the distribution and site location of Nippostrongylus brasiliensis within the small intestine of laboratory rats. Parasitology 61, 449–60.CrossRefGoogle ScholarPubMed
Alphey, T. J. W. (1971). Studies on the aggregation behaviour of Nippostrongylus brasiliensis. Parasitology 63, 109–17.Google Scholar
Bueding, E. (1949). Studies on the metabolism of the filarial worm, Litomosoides carinii. Journal of Experimental Medicine 89, 107–30.CrossRefGoogle Scholar
Crompton, D. W. T. (1970). An Ecological Approach to Acanthocephalan Physiology. Cambridge University Press.Google Scholar
Crompton, D. W. T., Shrimpton, D. H. & Silver, I. A. (1965). Measurements of the oxygen tension in the lumen of the small intestine of the domestic duck. Journal of Experimental Biology 43, 473–8.Google Scholar
Davey, D. G. (1938). The respiration of nematodes of the alimentary tract. Journal of Experimental Biology 15, 217–24.CrossRefGoogle Scholar
Hobson, A. D. (1948). The physiology and cultivation in artificial media of nematodes parasitic in the alimentary tract of animals. Parasitology 38, 183–227.CrossRefGoogle ScholarPubMed
Lee, D. L. (1969). Changes in adult Nippostrongylus brasiliensis during the development of immunity to this nematode in rats. I. Changes in ultrastructure. Parasitology 59, 29–39.CrossRefGoogle Scholar
Phillipson, R. F. (1966). Life cycle and chemotherapeutic studies on Nippostrongylus brasiliensis. Ph.D. Thesis, London School of Hygiene and Tropical Medicine.Google Scholar
Roberts, L. S. & Fairbairn, D. (1965). Metabolic studies on adult Nippostrongylus brasiliensis (Nematoda: Trichostrongyloidea). Journal of Parasitology 51, 129–39.CrossRefGoogle ScholarPubMed
Roche, M. & Torres, C. M. (1960). A method for in vitro study of Hookworm activity. Experimental Parasitology 9, 250–6.CrossRefGoogle ScholarPubMed
Rogers, W. P. (1949 a). Aerobic metabolism in nematode parasites of the alimentary tract. Nature, London. 163, 879–80.Google Scholar
Rogers, W. P. (1949 b). On the relative importance of aerobic metabolism in small nematode parasites of the alimentary tract. I. Oxygen tensions in the normal environment of the parasites. Australian Journal of Scientific Research, Series B 2, 157–65.Google Scholar
Santmyer, P. H. (1956). Studies on the metabolism of Panagrellus redivivus (Nematoda, Cephalobidae). Proceedings of the Helminthological Society of Washington 23, 30–6.Google Scholar