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An enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to Encephalitozoon cuniculi and its use in determination of infections in man

Published online by Cambridge University Press:  06 April 2009

Wafaa S. Hollister  and
Elizabeth U. Canning
Wafaa S. Hollister
Affiliation:
Department of Pure and Applied Biology, Imperial College of Science and Technology, London 8W7 2BB
Elizabeth U. Canning
Affiliation:
Department of Pure and Applied Biology, Imperial College of Science and Technology, London 8W7 2BB
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Summary

An enzyme-linked immunosorbent assay (ELISA) was used to detect antibodies to Encephalitozoon cuniculi in man, using spores derived from tissue culture as antigen. Negligible cross-reactions were found with other microsporidia of vertebrate or of invertebrate origin and there was no cross-reaction with Toxoplasma gondii, using ELISA, immunoperoxidase or immunofluorescence staining. A high prevalence of antibodies to E. cuniculi was found in patients suffering from schistosomiasis, malaria and neurological and psychiatric disorders, but not in healthy individuals.

Type
Research Article
Information
Parasitology , Volume 94 , Issue 2 , April 1987 , pp. 209 - 219
Copyright
Copyright © Cambridge University Press 1987

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