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An electrophysiological preparation of Ascaris suum pharyngeal muscle reveals a glutamate-gated chloride channel sensitive to the avermectin analogue, milbemycin D

Published online by Cambridge University Press:  26 March 2010

R. J. Martin
R. J. Martin
Affiliation:
Department of Preclinical Veterinary Sciences, R.(D.) S. V. S., Summerhall, University of Edinburgh, Edinburgh EH9 1QT
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Summary

An electrophysiological preparation of Ascaris suum pharyngeal muscle suitable for recording changes of input conductance using a 2-microelectrode current clamp and pharmacological study is described. The preparation is shown to contain a glutamate-gated Cl (ion sensitive) channel sensitive to the avermectin analogue, milbemycin D. The application of glutamate produces a dose-dependent increase in Cl conductance and the effect of glutamate is potentiated by milbemycin D. Milbemycin D also produced a dose-dependent increase in input conductance.

Keywords

glutamateavermectinmilbemycinchloride channelanthelminticAscaris suum
Type
Research Article
Information
Parasitology , Volume 112 , Issue 2 , February 1996 , pp. 247 - 252
Copyright
Copyright © Cambridge University Press 1996

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