Hostname: page-component-586b7cd67f-2plfb Total loading time: 0 Render date: 2024-11-24T08:31:17.230Z Has data issue: false hasContentIssue false
  • English
  • Français

An antibody-based ELISA for quantification of Ani s 1, a major allergen from Anisakis simplex

Published online by Cambridge University Press:  01 April 2008

M. C. ARILLA ,
I. IBARROLA ,
A. MARTÍNEZ ,
J. MONTESEIRÍN ,
J. CONDE  and
J. A. ASTURIAS
M. C. ARILLA
Affiliation:
Research & Development Department, Bial-Arístegui, Alameda Urquijo, 27, 48008 Bilbao, Spain
I. IBARROLA
Affiliation:
Research & Development Department, Bial-Arístegui, Alameda Urquijo, 27, 48008 Bilbao, Spain
A. MARTÍNEZ
Affiliation:
Research & Development Department, Bial-Arístegui, Alameda Urquijo, 27, 48008 Bilbao, Spain
J. MONTESEIRÍN
Affiliation:
Department of Medicine, Immunology and Allergy Service, Virgen Macarena University Hospital, Sevilla Sagrado Corazón Clinic, Sevilla, Spain
J. CONDE
Affiliation:
Sagrado Corazón Clinic, Sevilla, Spain
J. A. ASTURIAS*
Affiliation:
Research & Development Department, Bial-Arístegui, Alameda Urquijo, 27, 48008 Bilbao, Spain
*
*Corresponding author: Bial-Arístegui, R&D; Alameda Urquijo, 27; 48008-Bilbao, Spain. Tel: +34 944 438 000. Fax: +34 944 438 016. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Summary

Anisakis simplex is a nematode parasite that can infect humans who have eaten raw or undercooked seafood. Larvae invading the gastrointestinal mucosa excrete/secrete proteins that are implicated in the pathogenesis of anisakiasis and can induce IgE-mediated symptoms. Since Ani s 1 is a potent secreted allergen with important clinical relevance, its measurement could assess the quality of allergenic products used in diagnosis/immunotherapy of Anisakis allergy and track the presence of A. simplex parasites in fish foodstuffs. An antibody-based ELISA for quantification of Ani s 1 has been developed based on monoclonal antibody 4F2 as capture antibody and biotin-labelled polyclonal antibodies against Ani s 1 as detection reagent. The dose-response standard curves, obtained with natural and recombinant antigens, ranged from 4 to 2000 ng/ml and were identical and parallel to that of the A. simplex extract. The linear portion of the dose-response curve with nAni s 1 was between 15 and 250 ng/ml with inter-assay and intra-assays coefficients of variation less than 20% and 10%, respectively. The assay was specific since there was no cross-reaction with other extracts (except Ascaris extracts) and was highly sensitive (detection limit of 1·8 ng/ml), being able to detect Ani s 1 in fish extracts from codfish and monkfish.

Keywords

Anisakis simplexAnisakis allergyAni s 1quantificationmonoclonal antibodyimmunoassayfood allergyrecombinant antigenparasite detection
Type
Original Articles
Information
Parasitology , Volume 135 , Issue 6 , May 2008 , pp. 735 - 740
Copyright
Copyright © 2008 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Añibarro, B. and Seoane, F. J. (1998). Occupational conjunctivitis caused by sensitization to Anisakis simplex. Journal of Allergy and Clinical Immunology 102, 331–332.CrossRefGoogle ScholarPubMed
Arilla, M. C., Asturias, J. A., Gómez Bayón, N., Martínez, A., Martínez, J. and Palacios, R. (1997). Production and characterization of profilin monoclonal antibodies. Allergologie et Immunopathologie 25, 145–151.Google ScholarPubMed
Arilla, M. C., Ibarrola, I., Eraso, E., Aguirre, M., Martínez, A. and Asturias, J. A. (2001). Quantification in mass units of group 1 grass allergens by a monoclonal antibody-based sandwich ELISA. Clinical and Experimental Allergy 31, 1271–1278.CrossRefGoogle ScholarPubMed
Armentia, A., Lombardero, M., Callejo, A., Martín-Santos, J. M., Martín-Gil, F. J., Vega, J., Arranz, M. L. and Martínez, C. (1998). Occupational asthma by Anisakis simplex. Journal of Allergy and Clinical Immunology 102, 831–834.CrossRefGoogle ScholarPubMed
Armentia, A., Martín-Gil, F. J., Pascual, C., Martín-Esteban, M., Callejo, A. and Martínez, C. (2006). Anisakis simplex allergy after eating chicken meat. Journal of Investigational Allergology and Clinical Immunology 16, 258–263.Google ScholarPubMed
Asturias, J. A., Arilla, M. C., Aguirre, M., Gómez Bayón, N., Martínez, A., Palacios, R., Sánchez Gascón, F. and Martínez, J. (1999). Quantification of profilins by a monoclonal antibody-based sandwich ELISA. Journal Immunological Methods 229, 61–71.CrossRefGoogle ScholarPubMed
Audicana, M., Fernández de Corres, L., Muñoz, D., Fernández, E., Navarro, J. A. and Del Pozo, M. D. (1995). Recurrent anaphylaxis caused by Anisakis simplex parasitizing fish. Journal of Allergy and Clinical Immunology 96, 558–560.CrossRefGoogle ScholarPubMed
Baeza, M. L., Rodríguez, A., Matheu, V., Rubio, M., Tornero, P., de Barrio, M., Herrero, T., Santaolalla, M. and Zubeldía, J. M. (2004). Characterization of allergens secreted by Anisakis simplex parasite: clinical relevance in comparison with somatic allergens. Clinical and Experimental Allergy 34, 296–302.CrossRefGoogle ScholarPubMed
Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248–254.CrossRefGoogle ScholarPubMed
Caballero, M. L. and Moneo, I. (2002). Specific IgE determination to Ani s 1, a major allergen from Anisakis simplex, is a useful tool for diagnosis. Annual Allergy, Asthma and Immunology. 89, 74–77.CrossRefGoogle Scholar
Del Pozo, M. D., Audicana, M., Diez, J. M., Muñoz, D., Ansotegui, I. J., Fernández, E., García, M., Etxenagusia, M., Moneo, I. and de Corres, L. F. (1997). Anisakis simplex, a relevant etiologic factor in acute urticaria. Allergy 52, 576–579.CrossRefGoogle ScholarPubMed
Gómez-Aguado, F., Picazo, A., Caballero, M. L., Moneo, I., Asturias, J. A., Corcuera, M. T., Casado, I. and Alonso, M. J. (2003). Ultrastructural localization of Ani s 1, a major allergen from the fish parasite Anisakis simplex. Parasitology Research 89, 379–380.CrossRefGoogle Scholar
Ibarrola, I., Arilla, M. C., Herrero, M. D., Esteban, M. I., Martínez, A. and Asturias, J. A. (2008). Expression of a recombinant form immunochemically equivalent to the major Anisakis simplex allergen Ani s 1. Journal of Investigational Allergology and Clinical Immunology (in the Press).Google Scholar
Kennedy, M. W., Tierneym, J., Ye, P., McMonagle, F. A., McIntosh, A., McLaughlin, D. and Smith, J. W. (1988). The secreted and somatic antigens of the third stage larva of Anisakis simplex, and antigenic relationship with Ascaris suum, Ascaris lumbricoides, and Toxocara canis. Molecular and Biochemical Parasitology 31, 35–46.CrossRefGoogle ScholarPubMed
Laemmli, U. K. (1970). Cleavage of structural proteins during the assambly of the head of bacteriophage T4. Nature, London 277, 680–685.CrossRefGoogle Scholar
Levsen, A., Lunestad, B. T. and Berland, B. (2005). Low detection efficiency of candling as a commonly recommended inspection method for nematode larvae in the flesh of pelagic fish. Journal of Food Protection 68, 828–832.CrossRefGoogle ScholarPubMed
Lorenzo, S., Iglesias, R., Leiro, J., Ubeira, F. M., Ansotegui, I., García, M. and Fernández de Corres, L. (2000). Usefulness of currently available methods for the diagnosis of Anisakis simplex allergy. Allergy 55, 627–633.CrossRefGoogle ScholarPubMed
Lozano, M. J., Martín, H. L., Díaz, S. V., Mañas, A. I., Valero, L. A. and Campos, B. M. (2004). Cross-reactivity between antigens of Anisakis simplex s.l. and other ascarid nematodes. Parasite 11, 219–223.CrossRefGoogle ScholarPubMed
Moneo, I., Caballero, M. L., Gómez, F., Ortega, E. and Alonso, M. J. (2000). Isolation and characterization of a major allergen from the fish parasite Anisakis simplex. Journal of Allergy and Clinical Immunology 106, 177–181.CrossRefGoogle Scholar
Montoro, A., Peterguer, M. J., Chivato, T., Laguna, R. and Cuéllar, C. (1997). Recidivous acute urticaria caused by Anisakis simplex. Allergy 52, 985–991.CrossRefGoogle ScholarPubMed
Scala, E., Giani, M., Pirrotta, L., Guerra, E. C., Cadoni, S., Girardelli, C. R., De Pità, O. and Puddu, P. (2001). Occupational generalised urticaria and allergic airborne asthma due to Anisakis simplex. European Journal of Dermatology 11, 249–250.Google ScholarPubMed
Scala Pulido-Marrero, Z., González-Mancebo, E., Alfada-Arias, T., de le Hoz-Caballer, B. and Cuevas-Agustín, M. (2000). Unusual sensitization to Anisakis simplex. Allergy 55, 586–587.CrossRefGoogle Scholar
Valls, A., Pascual, C. Y. and Martín Esteban, M. (2003). Anisakis y anisakiosis. Allergologie et Immunopathologie 31, 348–355.CrossRefGoogle ScholarPubMed
van Ree, R. (2004). The create project: EU support for the improvement of allergen standardization in Europe Europe. Allergy 59, 571–574.CrossRefGoogle ScholarPubMed
Wharton, D. A., Hassall, M. L., Aalders, O. (1999). Anisakis (Nematodoa) in some New Zealand inshore fish. New Zealand Journal of Marine and Freshwater Research 33, 643–648.CrossRefGoogle Scholar