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The adaptation of three isolates of Babesia divergens to continuous culture in rat erythrocytes

Published online by Cambridge University Press:  06 April 2009

N. Ben Musa  and
R. S. Phillips
N. Ben Musa
Affiliation:
Wellcome Laboratories for Experimental Parasitology, Bearsden Road, University of Glasgow, Glasgow G61 1QH
R. S. Phillips
Affiliation:
Wellcome Laboratories for Experimental Parasitology, Bearsden Road, University of Glasgow, Glasgow G61 1QH
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Abstract

Three isolates of Babesia divergens have been cultured continuously for 6 months in rat erythrocytes using the candle jar technique (Trager & Jensen, 1976). One isolate was already rat-adapted, the other two became adapted to rats through continuous culture in rat erythrocytes. Parasites were cultured in rat erythrocytes in RPMI medium supplemented with 20% foetal calf serum. The highest parasitaemia obtained was 35% and multiparasitization of red blood cells was often observed. Cultures of B. divergens remained infective to splenectomized rats. Cultures with high parasitaemias contained a large number of extracellular merozoites. When separated from the red blood cells, these extracellular merozoites retained their infectivity.

Keywords

Babesia divergensin vitro cultureadaptation to rats
Type
Research Article
Information
Parasitology , Volume 103 , Issue 2 , October 1991 , pp. 165 - 170
Copyright
Copyright © Cambridge University Press 1991

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References

Canning, E. U. & Winger, C. M. (1987). Babesiidae. In In vitro methods for Parasite Cultivation (ed. Taylor, A. E. R. & Baker, J. R.) pp. 199–229. London: Academic Press.Google Scholar
Donnelly, J., Phipps, L. P. & Konrad, J. (1984). Infectivity in bovine hosts of Babesia divergens and Babesia major from in vitro culture. Parasitology 89, LXXV1 Abstract.Google Scholar
Gorenflot, A., Brasseur, P., Precigout, E., L'Hostis, M., Marchand, A. & Schrevel, J. (1991). Cytological and immunological responses to Babesia divergens in different hosts: ox, gerbil, man. Parasitology Research 77, 3–12.Google Scholar
Gray, G. D. & Phillips, R. S. (1981). Use of sorbitol in the cryopreservation of babesia. Research in Veterinary Science 30, 388–9.CrossRefGoogle ScholarPubMed
Konrad, J., Phipps, L. P., Canning, E. U. & Donnelly, J. (1984). Long term in vitro maintenance of Babesia divergens in a stationary phase culture. Parasitology 89, LXXVI Abstract.Google Scholar
Lewis, D. & Williams, H. (1979). Infection of the Mongolian gerbil with the cattle piroplasm Babesia divergens. Nature, London 278, 120–1.CrossRefGoogle ScholarPubMed
Phillips, R. S. (1984). Babesia divergens in splenectomized rats. Research in Veterinary Science 36, 251–5.Google Scholar
Phillips, R. S. & Wilson, R. J. M. (1978). Cryopreservation of Plasmodium falciparum in liquid nitrogen. Transactions of the Royal Society of Tropical Medicine and Hygiene 72, 643–4.Google Scholar
Phillips, R. S., Reid, G. M., McLean, S. A. & Pearson, C. D. (1987). Antigenic diversity in Babesia divergens: preliminary results with the three monoclonal antibodies to the rat-adapted strain. Research in Veterinary Science 42, 96–100.Google Scholar
Ristic, M. & Levy, M. G. (1981). A new era of research towards solution of bovine babesiosis. In Babesiosis (ed. Ristic, M. & Kreier, J. P.), pp. 509–544. New York: Academic Press.Google Scholar
Rodriguez, S. D. & Buening, G. M., Green, T. J. & Carson, C. A. (1983). The cloning of Babesia bigemina by in vitro cultivation. Infection and Immunity 42, 15–18.CrossRefGoogle Scholar
Trager, W. & Jensen, J. B. (1976). Human malaria parasites in continuous culture. Science 193, 673–5.Google Scholar
VÄYrynen, R. & Tuomi, J. (1982). Continuous in vitro cultivation of Babesia divergens. Acta Veterinaria Scandinavica 23, 471–2.Google Scholar
Vega, C. A., Buening, G. M., Rodriguez, S. D., Carson, C. A. & McLaughlin, K. (1985). Cryopreservation of Babesia bigemina for in vitro cultivation. American Journal of Veterinary Research 46, 421–3.Google Scholar
Walliker, D., Carter, R. & Morgan, S. (1971). Genetic recombination in malaria parasites. Nature, London 232, 561–2.CrossRefGoogle ScholarPubMed
Winger, M. C., Canning, E. U. & Culverhouse, J. D. (1987). A monoclonal antibody to Babesia divergens which inhibits merozoite invasion. Parasitology 94, 17–27.CrossRefGoogle ScholarPubMed