Hostname: page-component-586b7cd67f-2plfb Total loading time: 0 Render date: 2024-11-28T10:10:38.491Z Has data issue: false hasContentIssue false
  • English
  • Français

Activity of two extracts of Cynanchum paniculatum against Ichthyophthirius multifiliis theronts and tomonts

Published online by Cambridge University Press:  08 December 2016

WEN JI-HONG ,
WANG YAN-LI ,
LIU YU-HUA ,
ZHANG JI-YUAN  and
LI ZE-HONG
WEN JI-HONG
Affiliation:
First Hospital, Jilin University, Changchun, Jilin, China
WANG YAN-LI
Affiliation:
First Hospital, Jilin University, Changchun, Jilin, China
LIU YU-HUA
Affiliation:
Hospital of Harbin Engineering University, Harbin, China
ZHANG JI-YUAN
Affiliation:
College of optical And Electronical Information Changchun University of Science and Technology, Changchun, Jilin, China
LI ZE-HONG*
Affiliation:
Jilin Agriculture University, 2888 Xincheng Road, Changchun, Jilin, China
*
*Corresponding author. Agriculture University, 2888 Xincheng Road, Changchun, Jilin, China. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Summary

The present study aims to evaluate the antiparasitic activity of active components from Cynanchum paniculatum against Ichthyophthirius multifiliis. The antiparasitic activities of two bioassay-guided fractionationated compounds from C. paniculatum identified as Cynatratoside-A and Cynanversicoside C, by comparing spectral data (NMR and ESI-MS) with literature values, were evaluated by in vitro assay. These showed that both could kill theronts of I. multifiliis at a concentration of 10·0 mg L−1, with the median effective concentration (EC50) values of 4·6 mg L−1 and 5·2 mg L−1 for Cynatratoside-A and Cynanversicoside C, respectively. Encysted tomonts were killed at concentrations of 8·0 mg L−1 with both compounds. In vivo experiments demonstrated that fish treated with both compounds at 15·0 mg L−1 carried significantly fewer parasites than controls (P < 0·05). There were no mortalities among treated fish group compared with 75% mortality of untreated fish. Cynatratoside-A and Cynanversicoside C are therefore potential candidate drugs for use against I. multifiliis.

Keywords

Ichthyophthirius multifiliisCynatratoside-ACynanversicoside Cplant extracttreatmentCynanchum paniculatum
Type
Research Article
Information
Parasitology , Volume 144 , Issue 2 , February 2017 , pp. 179 - 185
Check for updates
Copyright
Copyright © Cambridge University Press 2016 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Bourgaud, F., Gravot, A., Milesi, E. and Gontier, E. (2001). Production of plant secondary metabolites: an historical perspective. Plant Science 161, 839–851.Google Scholar
Buchmann, K., Sigh, J., Nielsen, C. V. and Dalgaard, M. (2001). Host responses against the fish parasitizing ciliate Ichthyophthirius multifiliis . Veterinary Parasitology 100, 105–116.Google Scholar
Callinan, R. B. and Rowland, S. J. (1995) Diseases of silver perch. In Silver Perch Culture (ed. Rowland, S. J. and Bryant, C.), pp. 67–75. Austasia Aquaculture, Sandy Bay, Australia.Google Scholar
Chu, C., Zhang, Q. Z. and Luo, F. (2010). Effect of twenty Chinese herbal medicines on killing trophonts, cysts and theronts of Ichthyophthirius multifiliis in vitro . Freshwater Fishery 40, 55–60 (Chinese with English abstract).Google Scholar
Dickerson, H. W. (2006). Ichthyophthirius multifiliis and Cryptocary onirritans (Phylum). In Fish Diseases and Disorders. Protozoan and Metazoan Infections, vol. 1, 2nd Edn. (ed. Woo, P. T. K.), pp. 116–153. CAB International, Wallingford, UK.Google Scholar
Fu, Y. W., Zhang, Q. Z., Xu, D. H., Liang, J. H. and Wang, B. (2014). Antiparasitic effect of Cynatratoside-C from Cynanchu matratum against Ichthyophthirius multifiliis on Grass Carp. Journal of Agricultural and Food Chemistry 62, 7183–7189.Google Scholar
Jadhav, R. S., Ahmed, L., Swamy, P. L. and Sanaullah, S. (2013). Neuroprotective effects of polyhydroxy pregnane glycoside isolated from Wattakaka volubilis (L.f.) Stapf. after middle cerebral artery occlusion and reperfusion in rats. Brain Research 1515, 78–87.Google Scholar
Ling, F., Wang, J. G., Liu, Q. F., Li, M., Ye, L. T. and Gong, X. L. (2010). Prevention of Ichthyophthirius multifiliis infestation in goldfish (Carassius auratus) by potassium ferrate (VI) treatment. Veterinary Parasitology 168, 212–216.CrossRefGoogle ScholarPubMed
Ling, F., Wang, J. G., Wang, G. X. and Gong, X. N. (2011). Effect of potassium ferrate (VI) on survival and reproduction of Ichthyophthirius multifiliis tomonts. Parasitology Research 109, 1423–1428.Google Scholar
Liu, S., Chen, Z., Wu, J., Wang, L., Wang, H. and Zhao, W. (2013). Appetite suppressing pregnane glycosides from the roots of Cynanchum auriculatum . Phytochemistry 93, 144–153.Google Scholar
Matthews, R. A. (2005). Ichthyophthirius multifiliis Fouquet and ichthyophthiriosis in freshwater teleosts. Advance in Parasitology 59, 159–241.Google Scholar
Mimaki, Y., Nakamura, O., Sashida, Y., Koike, K., Nikaido, T., Ohmoto, T., Nishino, A., Satomi, Y. and Nishino, H. (1995). Structures of steroidal saponins from the tubers of Brodiaeacali fornica and their inhibitory activity on tumor promoter-induced phospholipid metabolism. Chemical & Pharmaceutical Bulletin 43, 971–976.Google Scholar
Peng, Y. R., Li, Y. B., Liu, X. D., Zhang, J. F. and Duan, J. A. (2008). Antitumor activity of C-21 steroidal glycosides from Cynanchum auriculatum Royle ex Wight. Phytomedicine 15, 1016–1020.CrossRefGoogle ScholarPubMed
Qiu, S. X., Zhang, Z. X. and Zhou, J. (1989). Steroidal glycosides from the root of Cynanchu mvesicolor . Phytochemistry 28, 3175–3178.Google Scholar
Rintamäki-Kinnunen, P., Rahkonen, M., Mannermaa-Keränen, A. L., Suo-malainen, L. R., Mykrä, H. and Valtonen, E. T. (2005). Treatment of ichthyophthiriasis after malachite green. I. Concrete tanks at salmonid farms. Disease of Aquatic Organisms 64, 69–76.Google Scholar
Rowland, S. J., Mifsud, C., Nixon, M., Read, P. and Landos, M. (2009). Use of formalin and copper to control ichthyophthiriosis in the Australian freshwater fish silver perch (Bidyanusbidyanus Mitchell). Aquaculture Research 40, 44–54.Google Scholar
Shan, X. F., Meng, Q. F., Kang, Y. H., Bian, Y., Gao, Y. H., Wang, W. L. and Qian, A. D. (2014). Isolation of active compounds frommethanol extracts of Toddalia asiatica against Ichthyophthirius multifiliis in goldfish (Carassius auratus). Veterinary Parasitology 199, 250–254.Google Scholar
Shinn, A. P., Picóncamacho, S. M., Bron, J. E., Conway, D., Yoon, G. H., Guo, F. C. and Taylor, N. G. H. (2012). The anti-protozoal activity of bronopolonthe key life-stages of Ichthyophthirius multifiliis Fouquet, 1876 (Cilio-phora). Veterinary Parasitology 186, 229–236.CrossRefGoogle Scholar
Straus, D. L. and Griffin, B. R. (2002). Efficacy of potassium permanganate in treating ichthyophthiriasis in channel catfish. Journal of Aquatic Animal Health 14, 145–148.Google Scholar
Tagboto, S. and Townson, S. (2001). Antiparasitic properties of medicinal plants and other naturally occurring products. Advance in Parasitology 50, 199–295.Google Scholar
Traxler, G. S., Richard, J. and McDonald, T. E. (1998). Ichthyophthirius multifiliis (Ich) epizootics in spawning sockeye salmon in British Columbia, Canada. Journal of Aquatic Animal Health 10, 143–151.Google Scholar
Wang, L., Yin, Z. Q., Zhang, Q. W., Zhang, X. Q., Zhang, D. M. and Liu, K. (2011). Five new C21 steroidal glycosides from Periploca sepium . Steroids 76, 7638–7643.Google Scholar
Wohllebe, S., Richter, P. and Häder, D. P. (2012). Chlorophyllin for the control of Ichthyophthirius multifiliis (Fouquet). Parasitology Research 111, 729–733.Google Scholar
Xu, D. H., Shoemaker, C. A. and Klesius, P. H. (2008). Effect of tricainemethane sulfonate on survival and reproduction of the fish ectoparasite Ichthyophthirius multifiliis . Parasitology Research 103, 979–982.Google Scholar
Yang, Q. X., Ge, Y. C., Huang, X. Y. and Sun, Q. Y. (2011). Cynanauriculoside C–E, three new antidepressant pregnane glycosides from Cynanchum auriculatum. Phytochemical Letter 4, 170–175.Google Scholar
Yao, J. Y., Shen, J. Y., Li, X. L., Xu, Y., Hao, G. J., Pan, X. Y., Wang, G. X. and Yin, W. L. (2010). Effect of sanguinarine from the leaves of Macleaya cordata against Ichthyophthirius multifiliis in grass carp (Ctenopharyngo donidella). Parasitology Research 107, 1035–1042.Google Scholar
Yao, J. Y., Zhou, Z. M., Li, X. L., Yin, W. L., Ru, H. S., Pan, X. Y., Hao, G. J., Xu, Y. and Shen, J. Y. (2011). Antiparasitic efficacy of dihydrosanguinarine and dihydrochelerythrine from Macleaya microcarpa against Ichthyophthirius multifiliis in richadsin (Squaliobarbus curriculus). Veterinary Parasitology 183, 8–13.Google Scholar
Yao, J. Y., Li, X. C., Li, G., Xu, Y., Ai, W. M. and Shen, J. Y. (2014). Anti-parasitic activities of specific bacterial extracellular products of Streptomyces griseus SDX-4 against Ichthyophthirius multifiliis . Parasitology Research 113, 3111–3117.CrossRefGoogle ScholarPubMed
Zhang, Q. Z., Xu, D. H. and Klesius, P. H. (2013). Evaluation of an antiparasitic compound extracted from Gallachinensis against fish parasite Ichthyophthirius multifiliis . Veterinary Parasitology 198, 45–53.CrossRefGoogle ScholarPubMed
Zheng, W., Yan, C. M., Zhang, Y. B., Li, Z. H., Li, Z. Q., Li, X. Y., Wang, Z. W., Wang, X. L., Chen, W. Q. and Yu, X. H. (2014). Antiparasitic efficacy of Gracillin and Zingibernsis newsaponin from Costus speciosus (Koen ex. Retz) Sm. against Ichthyophthirius multifiliis . Parasitology 142, 1–7.Google Scholar