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Vaccination of merino lambs against haemonchosis with membrane-associated proteins from the adult parasite

Published online by Cambridge University Press:  06 April 2009

E. A. Munn ,
T. S. Smith ,
M. Grahama ,
C. A. Greenwood ,
A. S. Tavernor  and
G. Coetzee
E. A. Munn
Affiliation:
Immunology Department, AFRC Institute of Animal Physiology and Genetics Research, Cambridge Research Station, Babraham, Cambridge CB2 4AT
T. S. Smith
Affiliation:
Immunology Department, AFRC Institute of Animal Physiology and Genetics Research, Cambridge Research Station, Babraham, Cambridge CB2 4AT
M. Grahama
Affiliation:
Immunology Department, AFRC Institute of Animal Physiology and Genetics Research, Cambridge Research Station, Babraham, Cambridge CB2 4AT
C. A. Greenwood
Affiliation:
Immunology Department, AFRC Institute of Animal Physiology and Genetics Research, Cambridge Research Station, Babraham, Cambridge CB2 4AT
A. S. Tavernor
Affiliation:
Immunology Department, AFRC Institute of Animal Physiology and Genetics Research, Cambridge Research Station, Babraham, Cambridge CB2 4AT
G. Coetzee
Affiliation:
Janssen Pharmaceutica Animal Health Division, Private bag X9, 1665 Olifantsfontein, RSA
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Summary

Groups of South African, farm-reared merino lambs about 4 months old were vaccinated with extracts of adult Haemonchus contortus enriched with H11, an integral membrane protein from the parasite's intestinal microvilli, or with proteins soluble in Tween 20. The lambs were challenged with 5000 infective 3rd-stage larvae. Compared to the adjuvant-injected controls, lambs vaccinated with 350 μg/kg liveweight of the H11-enriched extract showed an 89% reduction in parasite egg production and an 88% reduction in total worm burden at post-mortem 35 days post-challenge. The H11-enriched extract contained some protein also present in the Tween 20 extract. Lambs vaccinated with 600 μg/kg of protein soluble in Tween 20 showed a 40% reduction in faecal egg counts and 51% reduction in total worm numbers 35 days post-challenge. Animals injected with 20 μg/kg of a sub-fraction containing H11 obtained from the H11 extract, showed a 71% reduction in egg output and total worm numbers.

Keywords

Haemonchus contortusmerinosmembrane proteinsvaccination.
Type
Research Article
Information
Parasitology , Volume 106 , Issue 1 , January 1993 , pp. 63 - 66
Copyright
Copyright © Cambridge University Press 1993

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References

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