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Vaccination of lambs against infection with Taenia ovis using antigens collected during short-term in vitro incubation of activated T. ovis oncospheres

Published online by Cambridge University Press:  06 April 2009

M. D. Rickard  and
A. J. Adolph
M. D. Rickard
Affiliation:
Department of Paraclinical Sciences, University of Melbourne, Veterinary Clinical Centre, Princes Highway, Werribee, Victoria 3030, Australia
A. J. Adolph
Affiliation:
Department of Paraclinical Sciences, University of Melbourne, Veterinary Clinical Centre, Princes Highway, Werribee, Victoria 3030, Australia
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Extract

Activated oncospheres of Taenia ovis were incubated for 24 h or 48 h at 37.5°C in Medium 858 alone or in Medium 858 enriched with 20% lamb serum or foetal calf serum. Antigens contained in the supernatant medium from these incubations were concentrated, emulsified in Freund's incomplete adjuvant and injected intramuscularly into lambs. These lambs developed a very high level of immunity to subsequent challenge infection with T. ovis eggs.

Type
Research Article
Information
Parasitology , Volume 75 , Issue 2 , October 1977 , pp. 183 - 188
Copyright
Copyright © Cambridge University Press 1977

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References

Coman, B. J. & Rickard, M. D. (1975). The location of Taenia Pisiformis, Taenia ovis and Taenia hydatigena in the gut of the dog and its effect on net environmental contamination with ova. Zeitschrift für Parasitenkunde 47, 237–48.CrossRefGoogle ScholarPubMed
Farag, H., Bout, D. & Capron, A. (1975). Specific immuno-diagnosis of human hydatidosis by the enzyme linked immunosorbent assay (E.L.I.S.A.). Biomedicine 23, 276–8.Google Scholar
Gemmell, M. A. & Soulsby, E. J. L. (1968). The development of acquired immunity to tapeworms and progress towards active immunization with special reference to Echinococcus spp. Bulletin of the World Health Organization 39, 45–55.Google ScholarPubMed
Healy, G. M., Fisher, D. C. & Parker, R. C. (1955). Nutrition of animal cells in tissue culture. X. Synthetic medium No. 858. Proceedings of the Society for Experimental Biology and Medicine 89, 71–7.CrossRefGoogle ScholarPubMed
Heath, D. D. (1970). The developmental biology of larval cyclophyllidean cestodes in mammals. Ph.D. thesis, Australian National University, Canberra.Google Scholar
Heath, D. D. (1976). Resistance to Taenia pisiformis larvae in rabbits: immunization against infection using non-living antigens from in vitro culture. International Journal for Parasitology 6, 19–24.CrossRefGoogle ScholarPubMed
Heath, D. D. & Pavlov, P. (1975). The fate of Taenia taeniaeformis oncospheres in normal and passively protected rats. International Journal for Parasitology 5, 83–8.CrossRefGoogle ScholarPubMed
Heath, D. D. & Smyth, J. D. (1970). In vitro cultivation of Echinococcus granulosus, Taenia hydatigena, T. ovis, T. pisiformis and T. serialis from oncosphere to cystic larva. Parasitology 61, 329–43.CrossRefGoogle Scholar
Ito, A. (1975). In vitro oncospheral agglutination given by immune sera from mice infected, and rabbits injected, with eggs of Hymenolepis nana. Parasitology 71, 465–73.CrossRefGoogle ScholarPubMed
Mitchell, G. F., Goding, J. W. & Rickard, M. D. (1977). Studies on immune responses to larval cestodes in mice. I. Increased susceptibility of certain mouse strains and hypothymic mice to Taenia taeniaeformis and analysis of passive transfer of resistance with serum. Australian Journal of Experimental Biology and Medical Science(in the Press).CrossRefGoogle ScholarPubMed
Musoke, A. & Williams, J. F. (1975). The immunological response of the rat to infection with Taenia taeniaeformis. V. Sequence of appearance of protective immunoglobulins and the mechanism of action of 7Sβ2a antibodies. Immunology 29, 855–66.Google ScholarPubMed
Rickard, M. D. (1974). Hypothesis for the long term survival of Taenia pisiformis cysticerci in rabbits. Zeitschrift für Parasitenkunde 44, 203–9.CrossRefGoogle Scholar
Rickard, M. D. & Adolph, A. J. (1976). Vaccination of calves against Taenia saginata infection using a ‘Parasite-free’ vaccine. Veterinary Parasitology 1, 389–92.CrossRefGoogle Scholar
Rickard, M. D. & Bell, K. J. (1971 a). Successful Vaccination of lambs against infection with Taenia ovis using antigens produced during in vitro cultivation of the larval stages. Research in Veterinary Science 12, 401–2.CrossRefGoogle ScholarPubMed
Rickard, M. D. & Bell, K. J. (1971 b). Immunity produced against Taenia ovis and T. taeniaeformis infection in lambs and rats following in vivo growth of their larvae in filtration membrane diffusion chambers. Journal of Parasitology 57, 571–5.CrossRefGoogle Scholar
Rickard, M. D. & Coman, B. J. (1977). Studies on the fate of Taenia hydatigena and Taenia ovis larvae in rabbits, and cross-immunity with Taenia pisiformis larvae. International Journal for Parasitology (in the Press).CrossRefGoogle ScholarPubMed
Rickard, M. D. & Katiyar, J. C. (1976). Partial purification of antigens collected during in vitro cultivation of the larval stages of Taenia pisiformis. Parasitology 72, 269–79.CrossRefGoogle ScholarPubMed
Rickard, M. D. & Outteridge, P. M. (1974). Antibody and cell-mediated immunity in rabbits infected with larval stages of Taenia pisiformis. Zeitschrift für Parasitenkunde 44, 187–204.CrossRefGoogle ScholarPubMed
Rickard, M. D., White, J. B. & Boddington, E. B. (1976). Vaccination of lambs against infection with Taenia ovis. Australian Veterinary Journal 52, 209–14.CrossRefGoogle ScholarPubMed
Silverman, P. H. (1954). Studies on the biology of some tapeworms of the genus Taenia. I. Factors affecting hatching and activation of taeniid ova, and some criteria of their viability. Annals of Tropical Medicine and Parasitology 48, 207–15.CrossRefGoogle Scholar
Silverman, P. H. (1955). A technique for studying the in vitro effect of serum on activated taeniid hexacanth embryos. Nature, London 176, 598–9.CrossRefGoogle ScholarPubMed
Silverman, P. H. & Maneely, R. B. (1955). Studies on the biology of some tapeworms of the genus Taenia. III. The role of the secreting gland of the hexacanth embryo in the penetration of the intestinal mucosa of the intermediate host, and some of its histochemical reactions. Annals of Tropical Medicine and Parasitology 49, 326–30.CrossRefGoogle ScholarPubMed
Verster, A. (1969). A taxonomic revision of the genus Taenia Linnaeus 1758. Onderstepoort Journal of Veterinary Research 36, 3–58.Google ScholarPubMed