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Vaccination of eels (Anguilla japonica and Anguilla anguilla) against Anguillicola crassus with irradiated L3

Published online by Cambridge University Press:  27 February 2008

K. KNOPF  and
R. LUCIUS
K. KNOPF*
Affiliation:
Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Müggelseedamm 310, 12587 Berlin, Germany
R. LUCIUS
Affiliation:
Department of Molecular Parasitology, Humboldt-University Berlin, Philippstraße 13, Haus 14, 10115 Berlin, Germany
*
*Corresponding author: Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Müggelseedamm 310, 12587 Berlin, Germany. Tel: +49 30 64181637. Fax: +49 30 64181663. E-mail: [email protected]
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Summary

The original host of the swimbladder nematode Anguillicola crassus, the Japanese eel (Anguilla japonica) and the recently colonized European eel (Anguilla anguilla) were immunized with 40 irradiated (500 Gy) 3rd-stage larvae (L3) of this parasite and challenged with an infection of 40 normal L3. The immunization induced a significant reduction of the number of adult worms developing from the challenge infection in A. japonica, but not in A. anguilla. The induced resistance (calculated using the relation of the number of adult worms in immunized eels and in non-immunized control eels) in A. japonica was 87·3%±30·4%. Following a single infection, the percentage of adult worms found in A. japonica was lower as compared to A. anguilla, and the few adult worms were much smaller, revealing a lower susceptibility of A. japonica to A. crassus in comparison to A. anguilla. Both eel species developed an antibody response against A. crassus, but the level of antibody responses was not positively correlated with the protection against infection, suggesting that the antibody response is not a key element in resistance of eels against A. crassus. This study suggests that the original host of A. crassus is able to mount efficient protective immune responses against its parasite, whereas the newly acquired host seems to lack this ability.

Keywords

Anguillicola crassusAnguilla japonicaAnguilla anguillairradiated L3vaccinationresistanceantibody response
Type
Original Articles
Information
Parasitology , Volume 135 , Issue 5 , May 2008 , pp. 633 - 640
Copyright
Copyright © Cambridge University Press 2008

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References

REFERENCES

Abraham, D., Leon, O., Schnyder-Candrian, S., Wang, C. C., Galioto, A. M., Kerepesi, L. A., Lee, J. J. and Lustigman, S. (2004). Immunoglobulin E and eosinophil-dependent protective immunity to larval Onchocerca volvulus in mice immunized with irradiated larvae. Infection and Immunity 72, 810–817.CrossRefGoogle ScholarPubMed
Blanc, G., Bonneau, S., Biagianti, S. and Petter, A. J. (1992). Description of the larval stages of Anguillicola crassus (Nematoda, Dracunculoidea) using light and scanning electron microscopy. Aquatic Living Resources 5, 307–318.CrossRefGoogle Scholar
Buchmann, K., Østergaard, L. and Glamann, J. (1992). Affinity purification of antigen-specific serum immunoglobulin from the European eel (Anguilla anguilla). Scandinavian Journal of Immunology 36, 89–97.CrossRefGoogle ScholarPubMed
Haenen, O. L. M., van Wijngaarden, T. A. M. and Borgsteede, F. H. M. (1994). An improved method for the production of infective third-stage juveniles of Anguillicola crassus. Aquaculture 123, 163–165.CrossRefGoogle Scholar
Hartmann, S., Sereda, M. J., Sollwedel, A., Kalinna, B. and Lucius, R. (2006). A nematode allergen elicits protection under specific conditions. Vaccine 24, 3581–3590.CrossRefGoogle ScholarPubMed
Kerepesi, L. A., Leon, O., Lustigman, S. and Abraham, D. (2005). Protective immunity to the larval stages of Onchocerca volvulus is dependent on Toll-like receptor 4. Infection and Immunity 73, 8291–8297.CrossRefGoogle Scholar
Kirk, R. S. (2003). The impact of Anguillicola crassus on European eels. Fisheries Management and Ecology 10, 385–394.CrossRefGoogle Scholar
Knopf, K. (2006). The swimbladder nematode Anguillicola crassus in the European eel Anguilla anguilla and the Japanese eel Anguilla japonica: differences in susceptibility and immunity between a recently colonized host and the original host. Journal of Helminthology 80, 129–136.CrossRefGoogle ScholarPubMed
Knopf, K. and Mahnke, M. (2004). Differences in susceptibility of the European eel (Anguilla anguilla) and the Japanese eel (Anguilla japonica) to the swimbladder nematode Anguillicola crassus. Parasitology 129, 491–496.CrossRefGoogle Scholar
Knopf, K., Naser, K., van der Heijden, M. H. T. and Taraschewski, H. (2000). Humoral immune response of European eel Anguilla anguilla experimentally infected with Anguillicola crassus. Diseases of Aquatic Organisms 42, 61–69.CrossRefGoogle ScholarPubMed
Knopf, K., Würtz, J., Sures, B. and Taraschewski, H. (1998). Impact of low water temperature on the development of Anguillicola crassus in the final host Anguilla anguilla. Diseases of Aquatic Organisms 33, 143–149.CrossRefGoogle ScholarPubMed
Kuwahara, A., Niimi, A. and Itagaki, H. (1974). Studies on a nematode parasitic in the air bladder of the eel. I. Description of Anguillicola crassa n. sp. (Philometridae, Anguillicolidae). Japanese Journal of Parasitology 23, 275–279.Google Scholar
Lucius, R., Textor, G., Kern, A. and Kirsten, C. (1991). Acanthocheilonema viteae: vaccination of jirds with irradiation-attenuated stage-3 larvae and with exported larval antigens. Experimental Parasitology 73, 184–196.CrossRefGoogle ScholarPubMed
Moravec, F. and Taraschewski, H. (1988). Revision of the genus Anguillicola Yamaguti, 1935 (Nematoda: Anguillicolidae) of the swimbladder of eels, including descriptions of two new species, A. novaezelandiae sp. n. and A. papernai sp. n. Folia Parasitologica 35, 125–146.Google Scholar
Nielsen, M. E. (1999). An enhanced humoral immune response against the swimbladder nematode, Anguillicola crassus, in the Japanese eel, Anguilla japonica, compared with the European eel, A. anguilla. Journal of Helminthology 73, 227–232.CrossRefGoogle ScholarPubMed
Schrempf-Eppstein, B., Kern, A., Textor, G. and Lucius, R. (1997). Acanthocheilonema viteae: vaccination with irradiated L3 induces resistance in three species of rodents (Meriones unguiculatus, Mastomys coucha, Mesocricetus auratus). Tropical Medicine and International Health 2, 104–110.CrossRefGoogle ScholarPubMed
Sures, B., Knopf, K., Würtz, J. and Hirt, J. (1999). Richness and diversity of parasite communities in European eels Anguilla anguilla of the River Rhine, Germany, with special reference to helminth parasites. Parasitology 119, 323–330.CrossRefGoogle ScholarPubMed
Sures, B. and Streit, B. (2001). Eel parasite diversity and intermediate host abundance in the River Rhine, Germany. Parasitology 123, 185–191.CrossRefGoogle ScholarPubMed
van der Heijden, M. H. T, Rooijakkers, J. B. M. A., Booms, G. H. R., Rombout, J. H. W. M. and Boon, J. H. (1995). Production, characterization and applicability of monoclonal antibodies to European eel (Anguilla anguilla L., 1758) immunoglobulin. Veterinary Immunology and Immunopathology 45, 151–164.Google Scholar