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Trichodina heterodentata (Ciliophora) infestation on Prochilodus lineatus larvae: a host–parasite relationship study

Published online by Cambridge University Press:  17 December 2013

GUSTAVO MORAES RAMOS VALLADÃO ,
SÍLVIA UMEDA GALLANI ,
SANTIAGO BENITES DE PÁDUA ,
MAURÍCIO LATERÇA MARTINS  and
FABIANA PILARSKI
GUSTAVO MORAES RAMOS VALLADÃO
Affiliation:
LAPOA – Laboratory of Aquatic Organisms Pathology, Aquaculture Center of São Paulo State University (CAUNESP), Jaboticabal, Brazil
SÍLVIA UMEDA GALLANI
Affiliation:
LAPOA – Laboratory of Aquatic Organisms Pathology, Aquaculture Center of São Paulo State University (CAUNESP), Jaboticabal, Brazil
SANTIAGO BENITES DE PÁDUA
Affiliation:
LAPOA – Laboratory of Aquatic Organisms Pathology, Aquaculture Center of São Paulo State University (CAUNESP), Jaboticabal, Brazil
MAURÍCIO LATERÇA MARTINS
Affiliation:
AQUOS-Aquatic Organism Health Laboratory, Aquaculture Department, Federal University of Santa Catarina (UFSC), Florianópolis, Brazil
FABIANA PILARSKI*
Affiliation:
LAPOA – Laboratory of Aquatic Organisms Pathology, Aquaculture Center of São Paulo State University (CAUNESP), Jaboticabal, Brazil
*
* Corresponding author: LAPOA – Laboratory of Aquatic Organisms Pathology, Aquaculture Center of São Paulo State University (CAUNESP), Via Prof. Paulo Donato Castellane, s/n, CEP: 14884-900 Jaboticabal, SP, Brazil. E-mail: [email protected]
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Summary

Prochilodus lineatus is a freshwater fish species found in South America. It is common in aquaculture, but few studies regarding diseases of this fish have been performed. This study presents data of the occurrence of Trichodina heterodentata Duncan, 1977, as well as the pathological alterations detected by light and scanning electron microscopy (SEM). Twenty 20-day-old larvae were harvested from an earth pond and examined. Larvae showed erratic swimming on the pond edges and some had a whitish tegument. Larval smears were either impregnated with silver nitrate or stained with Giemsa stain to observe the taxonomic features of the ciliates. Five larvae were fixed in formalin solution for histopathological analysis, and another five specimens were fixed in glutaraldehyde for SEM. All larvae were diagnosed with a severe infestation by trichodinid T. heterodentata. Histological sections showed discrete hyperplasia of the gill filaments with subepithelial oedema of the secondary lamellae. In the SEM, suction areas were observed on the skin, gill and eye; corrosion and ulceration of the fins were associated with the bacterial presence of cocci on the lesions. This is the first report of T. heterodentata in P. lineatus that is responsible for an acute disease that culminates in larval mortality.

Keywords

fish diseasesectoparasiteTrichodinidaepathogenhistopathologyscanning electron microscopy
Type
Research Article
Information
Parasitology , Volume 141 , Issue 5 , April 2014 , pp. 662 - 669
Copyright
Copyright © Cambridge University Press 2013 

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