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Toxocariasis/cysticercosis seroprevalence in a long-term rural settlement, São Paulo, Brazil

Published online by Cambridge University Press:  14 April 2009

L. E. PRESTES-CARNEIRO ,
D. H. P. SOUZA ,
G. C. MORENO ,
C. TROIANI ,
V. SANTARÉM ,
S. C. S. ZAGO ,
N. A. MIGUEL ,
S. B. Z. FREITAS ,
R. FARIA  and
L. MARTINI
...Show all authors
L. E. PRESTES-CARNEIRO*
Affiliation:
Departamento de Imunologia, Universidade do Oeste Paulista, Rua José Bongiovani 700, Cidade Universitária, Presidente Prudente, 19050-680, Presidente Prudente, SP, Brazil
D. H. P. SOUZA
Affiliation:
Departamento de Imunologia, Universidade do Oeste Paulista, Rua José Bongiovani 700, Cidade Universitária, Presidente Prudente, 19050-680, Presidente Prudente, SP, Brazil
G. C. MORENO
Affiliation:
Departamento de Imunologia, Universidade do Oeste Paulista, Rua José Bongiovani 700, Cidade Universitária, Presidente Prudente, 19050-680, Presidente Prudente, SP, Brazil
C. TROIANI*
Affiliation:
Departamento de Imunologia, Universidade do Oeste Paulista, Rua José Bongiovani 700, Cidade Universitária, Presidente Prudente, 19050-680, Presidente Prudente, SP, Brazil
V. SANTARÉM
Affiliation:
Departamento de Imunologia, Universidade do Oeste Paulista, Rua José Bongiovani 700, Cidade Universitária, Presidente Prudente, 19050-680, Presidente Prudente, SP, Brazil
S. C. S. ZAGO
Affiliation:
Departamento de Imunologia, Universidade do Oeste Paulista, Rua José Bongiovani 700, Cidade Universitária, Presidente Prudente, 19050-680, Presidente Prudente, SP, Brazil
N. A. MIGUEL
Affiliation:
Departamento de Imunologia, Universidade do Oeste Paulista, Rua José Bongiovani 700, Cidade Universitária, Presidente Prudente, 19050-680, Presidente Prudente, SP, Brazil
S. B. Z. FREITAS
Affiliation:
Departamento de Imunologia, Universidade do Oeste Paulista, Rua José Bongiovani 700, Cidade Universitária, Presidente Prudente, 19050-680, Presidente Prudente, SP, Brazil
R. FARIA
Affiliation:
Departamento de Imunologia, Universidade do Oeste Paulista, Rua José Bongiovani 700, Cidade Universitária, Presidente Prudente, 19050-680, Presidente Prudente, SP, Brazil
L. MARTINI
Affiliation:
Departamento de Imunologia, Universidade do Oeste Paulista, Rua José Bongiovani 700, Cidade Universitária, Presidente Prudente, 19050-680, Presidente Prudente, SP, Brazil
G. RUBINSKY-ELEFANT
Affiliation:
Instituto de Medicina Tropical de São Paulo, Prédio II, Laboratório de Soroepidemiologia e Imunobiologia, Av. Dr. Enéas de Carvalho Aguiar, 470, 40 andar, Cerqueira César, 05403-000, São Paulo, SP, Brazil
A. IHA
Affiliation:
Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Av. Lineu Prestes 580, 05508-9000, São Paulo-SP, Brazil
A. J. VAZ
Affiliation:
Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Av. Lineu Prestes 580, 05508-9000, São Paulo-SP, Brazil
*
*Corresponding author: Departamento de Imunologia, Universidade do Oeste Paulista, Rua José Bongiovani 700, Cidade Universitária, 19050-680, Presidente Prudente, São Paulo, Brazil. Tel/Fax: +55 18 229 1013. E-mail: [email protected]
*Corresponding author: Departamento de Imunologia, Universidade do Oeste Paulista, Rua José Bongiovani 700, Cidade Universitária, 19050-680, Presidente Prudente, São Paulo, Brazil. Tel/Fax: +55 18 229 1013. E-mail: [email protected]
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Summary

Seroprevalence of Toxocara and Taenia solium and risk factors for infection with these parasites were explored in a long-term rural settlement in São Paulo state, Brazil. An ELISA for the detection of anti-Toxocara IgG and IgE and anti-T. solium cysticerci was standardized using Toxocara excretory-secretory antigens (TES) obtained from the cultured second-stage larvae of T. canis and by vesicular fluid antigen from Taenia crassiceps cysticerci (VF). For cysticercosis, the reactive ELISA samples were assayed by Western blot using 18 kDa and 14 kDa proteins purified from VF. Out of 182 subjects, 25 (13·7%) presented anti-Toxocara IgG and a positive correlation between total IgE and the reactive index of specific anti-TES IgE (P=0·0265) was found amongst the subjects found seropositive for anti-Toxocara IgG. In these individuals 38·0% showed ocular manifestations. The frequency of anti-T. solium cysticerci confirmed by Western blot was 0·6%. Seropositivity for Toxocara was correlated with low educational levels and the owning of dogs. Embryonated eggs of Toxocara spp. were found in 43·3% of the analysed areas.

Keywords

ToxocaracysticercosisseroprevalenceELISABrazil
Type
Research Article
Information
Parasitology , Volume 136 , Issue 6 , May 2009 , pp. 681 - 689
Copyright
Copyright © Cambridge University Press 2009

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