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Short-interval absorption and metabolism of some amino acids in Trypanosoma gambiense*

Published online by Cambridge University Press:  06 April 2009

L. H. Chappell ,
G. C. Southworth  and
C. P. Read
L. H. Chappell
Affiliation:
Biology Department, Rice University, Houston, Texas 77001, U.S.A.
G. C. Southworth
Affiliation:
Biology Department, Rice University, Houston, Texas 77001, U.S.A.
C. P. Read
Affiliation:
Biology Department, Rice University, Houston, Texas 77001, U.S.A.
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Extract

During 2 min incubations of Trypanosoma gambiense (bloodstream form) with [U-14C]glucose (1 mM) over 60% of absorbed label was detected in free alanine. In the presence of 12·5 mM unlabelled alanine, the amount of alanine synthesized from glucose was reduced by less than 10%. These data support previous observations on the high level of transaminase activity in African human trypanosomes.

Alanine, aspartate and glutamate were metabolized to various other free amino acids whereas a significant amount of label derived from [14C]arginine could not be accounted for by amino acid assay.

The sulphur containing amino acids, cysteic acid and taurine, were apparently synthesized from alanine, glutamate and arginine. The significance of these syntheses is poorly understood.

Following incubations of trypanosomes for 2 min in exogenous amino acids the internal free pool became imbalanced due to accumulation and metabolism of the substrate amino acid.

Evidence obtained indicated that the level of free endogenous glutamate may be rate limiting for the glutamate-pyruvate transaminase system.

Type
Research Article
Information
Parasitology , Volume 64 , Issue 3 , June 1972 , pp. 379 - 387
Copyright
Copyright © Cambridge University Press 1972

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References

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