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A rhoptry antigen of Plasmodium falciparum is protective in Saimiri monkeys

Published online by Cambridge University Press:  06 April 2009

R. G. Ridley ,
B. Takacs ,
H. Etlinger  and
J. G. Scaife
R. G. Ridley
Affiliation:
Department of Molecular Biology, University of Edinburgh, King's Buildings, Mayfield Road, Edinburgh EH9 3JR, Switzerland
B. Takacs
Affiliation:
Central Research Units, F Hoffman-La Roche Limited, CH-4002 Basel, Switzerland
H. Etlinger
Affiliation:
Central Research Units, F Hoffman-La Roche Limited, CH-4002 Basel, Switzerland
J. G. Scaife
Affiliation:
Department of Molecular Biology, University of Edinburgh, King's Buildings, Mayfield Road, Edinburgh EH9 3JR, Switzerland
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Extract

A non-polymorphic antigen associated with the rhoptry organelles of Plasmodium falciparum has been purified by immuno-affinity chromatography. The antigen, RAP-1 (rhoptry associated protein-1). which is defined by monoclonal antibodies which inhibit parasite growth in vitro, is a multi-component antigen consisting of four major proteins of 80, 65, 42 and 40 kDa and two minor proteins of 77 and 70 kDa. These proteins were electro-eluted from preparative sodium dodecyl sulphate polyacrylamide gels and protected Saimiri sciureus monkeys from a lethal blood-stage infection of P. falciparum malaria. Sera from the protected animals recognized only proteins of the RAP-1 antigen when used to probe a Western blot of total parasite protein extract, confirming that RAP-1 is responsible for eliciting the protective immune response.

Keywords

Plasmodium falciparum Saimiri sciureus monkeysrhoptry antigenprotection
Type
Research Article
Information
Parasitology , Volume 101 , Issue 2 , October 1990 , pp. 187 - 192
Copyright
Copyright © Cambridge University Press 1990

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