Hostname: page-component-586b7cd67f-rdxmf Total loading time: 0 Render date: 2024-11-30T19:00:21.791Z Has data issue: false hasContentIssue false

RFLP analysis of PCR-amplified small subunit ribosomal DNA of three fish microsporidian species

Published online by Cambridge University Press:  01 February 2000

J. LEIRO
Affiliation:
Laboratorio de Parasitología, Facultad de Farmacia, Universidad de Santiago de Compostela, 15706 Santiago de Compostela, Spain
M. I. G. SISO
Affiliation:
Departamento de Biología Celular y Molecular, Laboratorio de Bioquímica, Universidad de La Coruña, , Campus da Zapateira s/n, La Coruña, Spain
A. PARAMA
Affiliation:
Laboratorio de Parasitología, Facultad de Farmacia, Universidad de Santiago de Compostela, 15706 Santiago de Compostela, Spain
F. M. UBEIRA
Affiliation:
Laboratorio de Parasitología, Facultad de Farmacia, Universidad de Santiago de Compostela, 15706 Santiago de Compostela, Spain
M. L. SANMARTIN
Affiliation:
Instituto de Investigación y Análisis Alimentarios, Laboratorio de Parasitología, Facultad de Farmacia, Universidad de Santiago de Compostela, 15706 Santiago de Compostela, Spain

Abstract

The phylogenetic relationships of the microsporidian species Microgemma caulleryi, Pleistophora finisterrensis and Tetramicra brevifilum were investigated on the basis of restriction fragment length polymorphism (RFLP) analysis of PCR-amplified small-subunit rDNA (SSUrDNA). Using PCR primers specific for microsporidian SSUrDNA, a single product was obtained from each species, and heteroduplex analysis indicated a high degree of sequence homology among the 3 products. In RFLP analysis of the PCR-amplified SSUrDNA, the enzymes AluI and DdeI gave restriction patterns that differed among all 3 species. Phylogenetic analysis using restriction patterns as differential characters indicated that Microgemma caulleryi and Tetramicra brevifilum are more closely related to each other than to Pleistophora finisterrensis.

Type
Research Article
Copyright
2000 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)