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Release of radio-isotope labelled antigens from Plasmodium knowlesi during merozoite re-invasion in vitro

Published online by Cambridge University Press:  06 April 2009

A. A. McColm  and
P. I. Trigg
A. A. McColm
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA
P. I. Trigg
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA
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Summary

Labelled antigens sharing determinants with both membrane and cytoplasmic fractions of Plasmodium knowlesi were detected in culture medium after in vitro incubation of schizont-stage parasites previously pulse-labelled with [3H]isoleucine. Release of antigens occurred only during schizont rupture and merozoite re-invasion. Antigenic material accounted for up to a third of the total trichloroacetic acid-insoluble radioactivity released by the cells. Absorption experiments indicated that approximately two-thirds of this antibody-precipitable material shared determinants with parasite membrane antigens, with a similar quantity sharing determinants with cytoplasmic proteins. Using antisera derived against 2 different antigenic variants of P. knowlesi, no evidence for the release of variant-specific antigens was obtained. Centrifugal analysis revealed that the majority of the radio-isotope labelled antigens were particulate in nature; however, some appeared to exist in true solution.

Type
Research Article
Information
Parasitology , Volume 81 , Issue 1 , August 1980 , pp. 199 - 209
Copyright
Copyright © Cambridge University Press 1980

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