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Propagation of Plasmodium berghei and P. vinckei in mouse erythrocytes injected into chick embryos

Published online by Cambridge University Press:  06 April 2009

Frank Hawking  and
Kenneth Gammage
Frank Hawking
Affiliation:
National Institute for Medical Research, London, N. W. 7
Kenneth Gammage
Affiliation:
National Institute for Medical Research, London, N. W. 7
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Extract

Mouse blood infected with Plasmodium berghei berghei, P. vinckei vinckei or P. vinckei chabaudi, was injected intravenously into 10-day-old chick embryos, and 4 days later further mouse erythrocytes were injected; after 7 days the infection was passed to another embryo with more mouse erythrocytes, and so on. By this procedure, P. berghei was propagated for 39 days through 6 embryos, P. vinckei vinckei for over 45 days through 7 embryos, and P. vinckei chabaudi for over 67 days through 10 embryos. The plasmodia multiplied only in mouse erythrocytes; they invaded a few avian erythrocytes but did not proceed to schizogony in them. P. falciparum, P. knowlesi and P. cynomolgi did not survive in chick embryos longer than 2–3 days. Babesia rodhaini was maintained by this procedure for 9 days through 2 embryos.

Type
Research Article
Information
Parasitology , Volume 61 , Issue 1 , August 1970 , pp. 65 - 70
Copyright
Copyright © Cambridge University Press 1970

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References

REFERENCES

Gammage, K. & Hawking, F. (1968). Plasmodium berghei developing in mouse erythrocytes inside chick embryos. Transactions of the Royal Society of Tropical Medicine and Hygiene 62, 461.Google Scholar
Gammage, K. & Hawking, F. (1969). Plasmodium berghei and P. vinckei grown in mouse erythrocytes inside chick embryos. Transactions of the Royal Society of Tropical Medicine and Hygiene 63, 415.Google Scholar
Hawking, F. & Gammage, K. (1970). The timing of the asexual cycles of Plasmodium lophurae and of P. cathemerium. Journal of Parasitology 56, 17–26.CrossRefGoogle ScholarPubMed
Le Page, R. W. F. (1967). Short term cultivation of Trypanosoma brucei in vitro at 37°C. Nature 216, 1141–2.CrossRefGoogle Scholar
McGhee, R. B. (1949). Infection of mammalian erythrocytes by the avian malaria parasite, Plasmodium lophurae. Proceedings of the Society for Experimental Biology and Medicine 71, 92–3.CrossRefGoogle ScholarPubMed
McGhee, R. B. (1954). The infection of duck and goose erythrocytes by the mammalian parasite, Plasmodium berghei. Journal of Protozoology 1, 145–8.CrossRefGoogle Scholar