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Prevalence, hematological findings and genetic diversity of Bartonella spp. in domestic cats from Valdivia, Southern Chile

Published online by Cambridge University Press:  12 December 2016

ANANDA MÜLLER Open the ORCID record for ANANDA MÜLLER [Opens in a new window] ,
ROMINA WALKER ,
PEDRO BITTENCOURT ,
ROSANGELA ZACARIAS MACHADO ,
JYAN LUCAS BENEVENUTE ,
RENAN BRESSIANI DO AMARAL ,
LUIZ RICARDO GONÇALVES  and
MARCOS ROGÉRIO ANDRÉ
ANANDA MÜLLER*
Affiliation:
Facultad de Ciencias Veterinarias, Instituto de Ciencias Clínicas Veterinarias, Universidad Austral de Chile, Valdivia, Chile
ROMINA WALKER
Affiliation:
Facultad de Ciencias Veterinarias, Instituto de Ciencias Clínicas Veterinarias, Universidad Austral de Chile, Valdivia, Chile
PEDRO BITTENCOURT
Affiliation:
Facultad de Ciencias Veterinarias, Instituto de Ciencias Clínicas Veterinarias, Universidad Austral de Chile, Valdivia, Chile
ROSANGELA ZACARIAS MACHADO
Affiliation:
Departamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista (FCAV/UNESP), Jaboticabal, SP, Brazil
JYAN LUCAS BENEVENUTE
Affiliation:
Departamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista (FCAV/UNESP), Jaboticabal, SP, Brazil
RENAN BRESSIANI DO AMARAL
Affiliation:
Departamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista (FCAV/UNESP), Jaboticabal, SP, Brazil
LUIZ RICARDO GONÇALVES
Affiliation:
Departamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista (FCAV/UNESP), Jaboticabal, SP, Brazil
MARCOS ROGÉRIO ANDRÉ
Affiliation:
Departamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista (FCAV/UNESP), Jaboticabal, SP, Brazil
*
*Corresponding author: A. Müller, Facultad de Ciencias Veterinarias, Instituto de Ciencias Clínicas Veterinarias, Universidad Austral de Chile, Veterinary Hospital UACh, PO Box 567, Valdivia, Chile. E-mail: [email protected]
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Summary

The present study determined the prevalence, hematological findings and genetic diversity of Bartonella spp. in domestic cats from Valdivia, Southern Chile. A complete blood count and nuoG gene real-time quantitative PCR (qPCR) for Bartonella spp. were performed in 370 blood samples from cats in Valdivia, Southern Chile. nuoG qPCR-positive samples were submitted to conventional PCR for the gltA gene and sequencing for species differentiation and phylogenetic analysis. Alignment of gltA gene was used to calculate the nucleotide diversity, polymorphic level, number of variable sites and average number of nucleotide differences. Bartonella DNA prevalence in cats was 18·1% (67/370). Twenty-nine samples were sequenced with 62·0% (18/29) identified as Bartonella henselae, 34·4% (10/29) as Bartonella clarridgeiae, and 3·4% (1/29) as Bartonella koehlerae. Bartonella-positive cats had low DNA bacterial loads and their hematological parameters varied minimally. Each Bartonella species from Chile clustered together and with other Bartonella spp. described in cats worldwide. Bartonella henselae and B. clarridgeiae showed a low number of variable sites, haplotypes and nucleotide diversity. Bartonella clarridgeiae and B. koehlerae are reported for the first time in cats from Chile and South America, respectively.

Keywords

Bartonella henselaeBartonella clarridgeiaeBartonella koehleraecat scratch diseaseqPCRSouth America
Type
Research Article
Information
Parasitology , Volume 144 , Issue 6 , May 2017 , pp. 773 - 782
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Copyright
Copyright © Cambridge University Press 2016 

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