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Pomphorhynchus laevis (Acanthocephala): elevation of haemolymph protein concentrations in the intermediate host, Gammarus pulex (Crustacea: Amphipoda)

Published online by Cambridge University Press:  06 April 2009

C. R. Bentley  and
H. Hurd
C. R. Bentley
Affiliation:
Parasitology Research Group, Centre for Applied Entomology and Parasitology, Department of Biological Sciences, Keele University, Staffordshire ST5 5BG
H. Hurd
Affiliation:
Parasitology Research Group, Centre for Applied Entomology and Parasitology, Department of Biological Sciences, Keele University, Staffordshire ST5 5BG
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Summary

Comparisons were made between the protein concentrations and protein profiles of uninfected Gammarus pulex and those infected with cystacanths of Pomphorhynchus laevis. Comparisons of the extractable haemolymph volumes from uninfected and infected G. pulex, when related to host tissue weight, showed that there was no difference in the host tissue/haemolymph volume ratio between infected and uninfected individuals. SDS-PAGE of haemolymph samples resolved 36 bands of protein, the major components being band 6(19 kDa) and 21(33 kDa). Densitometric analysis of haemolymph samples showed that infected gammarids had total protein concentrations elevated by 84%. The (88 kDa) subunit was identified as the monomer of the respiratory protein haemocyanin. This band was significantly elevated in infected individuals (one-way ANOVA P < 0.001); increasing from 30 to 45% of the total haemolymph proteins.

Keywords

acanthocephalanamphipodGammarus pulexhaemocyaninhaemolymphPomphorhynchus laevis
Type
Research Article
Information
Parasitology , Volume 107 , Issue 2 , August 1993 , pp. 193 - 198
Copyright
Copyright © Cambridge University Press 1993

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