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Pairing and aggregation of Amblosoma suwaense (Trematoda: Brachylaimidae) metacercariae in vitro and partial characterization of lipids involved in chemo-attraction

Published online by Cambridge University Press:  06 April 2009

B. Fried  and
G. A. Robinson
B. Fried
Affiliation:
Department of Biology, Lafayette College, Easton, Pennsylvania 18042
G. A. Robinson
Affiliation:
Department of Biology, Lafayette College, Easton, Pennsylvania 18042
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Summary

Histochemical and thin-layer chromatographic (t.l.c.) analyses were made on neutral lipids in the free (unencysted) metacercariae of Amblosoma suwaense (Brachylaimidae). As determined by t.l.c. the major neutral lipid fractions in metacercariae removed directly from Campeloma decisum snails were free sterols and sterol esters. Metacercariae incubated for 1 h at 37±1° C in sterile Locke's solution released mainly sterol esters and a lesser amount of free sterols into the medium. As determined by Oil Red O (ORO) staining, metacercariae accumulated neutral lipid in the intestinal caeca during incubation and the excretory system was ORO negative. Behavioural studies showed that metacereariae paired and aggregated in vitro and were attracted to lipophilic but not to hydrophilic worm products. Following t.l.c. preparative analysis it was demonstrated that metacercariae were attracted to sterol ester worm products but not to free sterol products.

Type
Research Article
Information
Parasitology , Volume 82 , Issue 2 , April 1981 , pp. 225 - 229
Copyright
Copyright © Cambridge University Press 1981

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