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Oxytetracycline inhibition of mitochondrial protein synthesis in bovine lymphocytes infected with Theileria parva or stimulated by mitogen

Published online by Cambridge University Press:  06 April 2009

P. R. Spooner
P. R. Spooner
Affiliation:
International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya
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Summary

Oxytetracycline (OTC) significantly inhibited cytochrome c oxidase activity in bovine lymphocytes infected with Theileria parva and in uninfected mitogen-stimulated lymphocytes. The inhibitory effect was detected in vitro within 24 h of treatment with drug concentrations as low as 1 µg/ml. Following mitogen stimulation of lymphocytes, concentrations of 3 and 10 µg/ml OTC completely inhibited an increase in cytochrome c oxidase activity for 48–72 h. This inhibitory activity was considered to be due to a direct effect on lymphoblast mitochondrial protein synthesis. As a consequence, adenosine triphosphate activity was significantly reduced in lymphocytes stimulated either by infection with T. parva sporozoites or by mitogen and then treated with OTC. The results also indicated that parasite mitochondrial protein synthesis was inhibited by OTC. The activity of OTC reported in this study could explain the suppression of disease following ‘infection and treatment’ immunization against East Coast fever and the in vitro drug-inhibition of schizont development.

Keywords

Theileria parvaEast Coast feveroxytetracyclinemitochondrial protein synthesisbovine lymphocytesimmunization
Type
Research Article
Information
Parasitology , Volume 101 , Issue 3 , December 1990 , pp. 387 - 393
Copyright
Copyright © Cambridge University Press 1990

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