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Oncospheral penetration glands are the source of the EG95 vaccine antigen against cystic hydatid disease

Published online by Cambridge University Press:  21 July 2010

A. JABBAR ,
D. J. JENKINS ,
S. CRAWFORD ,
A. K. WALDUCK ,
C. G. GAUCI  and
M. W. LIGHTOWLERS
A. JABBAR*
Affiliation:
Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia
D. J. JENKINS
Affiliation:
School of Animal and Veterinary Sciences, Charles Sturt University, Locked Bag 588, Wagga Wagga, New South Wales 2678, Australia
S. CRAWFORD
Affiliation:
School of Botany, The University of Melbourne, Parkville, Victoria 3010, Australia
A. K. WALDUCK
Affiliation:
Department of Microbiology and Immunology, The University of Melbourne, Parkville, Victoria 3010, Australia
C. G. GAUCI
Affiliation:
Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia
M. W. LIGHTOWLERS
Affiliation:
Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia
*
*Corresponding author: Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia. Tel: +61 3 9731 2022. Fax: +61 3 9741 5461. E-mail: [email protected]
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Summary

Immunohistochemistry and immunogold labelling techniques were used to localize the EG95 vaccine antigen in Echinococcus granulosus oncospheres. In non-activated oncospheres, the cytoplasm of 2 pairs of bilateral cells exhibited specific positive labelling for the presence of EG95. No surface localization was seen in non-activated or recently activated oncospheres. Besides the staining of 2 pairs of bilateral cells, there was also a generalized distribution of specific staining for EG95 throughout the parenchyma of activated oncospheres. Immunogold labelling of non-activated oncosphere revealed specific reactivity for EG95 involving 2 pairs of bilateral cells and the ultrastructural characteristics of these cells were consistent with them being penetration gland cells. No other oncospheral structures stained specifically for the presence of EG95. The absence of surface location of EG95 in oncospheres suggests that the parasite may not be susceptible to vaccine-induced antibody and complement mediated attack until some post-oncospheral development has occurred. Further studies would be required to determine when the EG95 antigen associates with the parasite's surface, thus making them susceptible to immune attack.

Keywords

hydatid diseasevaccinationantigenEG95oncosphereslocation
Type
Research Article
Information
Parasitology , Volume 138 , Issue 1 , January 2011 , pp. 89 - 99
Copyright
Copyright © Cambridge University Press 2010

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