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Ocular toxoplasmosis in mice: comparison of two routes of infection

Published online by Cambridge University Press:  23 May 2005

R. C. TEDESCO ,
R. L. SMITH ,
S. CORTE-REAL  and
K. S. CALABRESE
R. C. TEDESCO
Affiliation:
Departamento de Ultra-estrutura e Biologia Celular, Laboratório de Biologia Estrutural do Instituto Oswaldo Cruz – Fundação Oswaldo Cruz, Avenida Brasil, 4365 CEP: 21045-900, Rio de Janeiro – RJ, Brasil Departamento de Morfologia, Disciplina de Anatomia Topográfica e Descritiva da Universidade Federal de São Paulo, Escola Paulista de Medicina, Rua Botucatu, 740 CEP 04023-900, São Paulo-SP, Brasil
R. L. SMITH
Affiliation:
Departamento de Morfologia, Disciplina de Anatomia Topográfica e Descritiva da Universidade Federal de São Paulo, Escola Paulista de Medicina, Rua Botucatu, 740 CEP 04023-900, São Paulo-SP, Brasil
S. CORTE-REAL
Affiliation:
Departamento de Ultra-estrutura e Biologia Celular, Laboratório de Biologia Estrutural do Instituto Oswaldo Cruz – Fundação Oswaldo Cruz, Avenida Brasil, 4365 CEP: 21045-900, Rio de Janeiro – RJ, Brasil
K. S. CALABRESE
Affiliation:
Departamento de Protozoologia, Laboratório de Imunomodulação do Instituto Oswaldo Cruz – Fundação Oswaldo Cruz Avenida Brasil, 4365 CEP: 21045-900, Rio de Janeiro – RJ, Brasil
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Abstract

This paper aims to test the influence of route of infection (intravitreal and instillation) on the course of ocular toxoplasmosis in mice, using the Toxoplasma gondii Me-49 strain. All mice inoculated intravitreally or by instillation presented the same pattern of infection. Using either route, parasites were observed in the retinal vessel with the formation of a glial reaction in the inner plexiforme layer and discontinuity of the pigmented epithelium of the retina 7 days after infection. However, when the intravitreal route was used a more intense inflammatory infiltrate was observed in the retina. The results suggest that inoculation route remarkably influences the inflammatory pattern in ocular toxoplasmosis and that the instillation route should be preferentially used in experimental infections in the murine ocular model of infection by T. gondii, specially with small animals where there is extensive needle damage, which is not observed in the instillation route.

Keywords

Toxoplasma gondiiocular toxoplasmosisintravitrealinstillationmouse model
Type
Research Article
Information
Parasitology , Volume 131 , Issue 3 , September 2005 , pp. 303 - 307
Copyright
© 2005 Cambridge University Press

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